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Title: Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm 
Author: Bozkurt, Yusuf; Akcay, Ergun; Tekin, Necmettin; Secer, Selcuk
Date: 2005
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Bozkurt, Y., Akcay, E., Tekin, N., & Secer, S. (2005). Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of Aquaculture - Bamidgeh, 57(2), 125-130.
Abstract: Rainbow trout (Oncorhynchus mykiss) sperm was frozen in liquid nitrogen in straws or as pellets on dry ice and stored in liquid nitrogen until artificial insemination. Sperm was diluted with one of three extenders containing 15% DMSO or 15% DMA as a cryoprotectant at a ratio of one part sperm to two parts extender. The straws were thawed in a water bath while the frozen pellets were thawed in their own extenders at 30oC for 30 s. For both freezing methods, the best fertil- ization results were obtained with extender III containing 15% DMSO, 52.3% (56.3% of the con- trol) for sperm frozen in straws and 48.4% (52.4% of the control) for sperm frozen as pellets. The interaction between extender and cryoprotectant was statistically significant (p<0.05).
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 6 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19138
Keywords: cryopreservation, extender, fertilization, Oncorhynchus mykiss, pellet, sperm, straw
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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