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Parentage Assignment in Striped Catfish (Pangasianodon hypophthalmus) Based on Microsatellite Marke

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Item Summary Nguyen M.T Le Nguyen T.P Nguyen T.L Duong A.L 2019-08-12T18:37:21Z 2019-08-12T18:37:21Z 2019
dc.identifier.issn 0792-156X
dc.description.abstract In this study, five microsatellite markers from two multiplex PCR sets were used to determine the pedigree of five striped catfish (Pangasianodon hypophthalmus) families that came from a breeding program in Vietnam. The number of alleles per locus and polymorphic information content (PIC) were 4 to 7 and 0.551-0.803, respectively. In the parentage analysis based on five microsatellites, 62.7% of offspring could be allocated unambiguously to their parental pairs using the perfect mismatch setting, and assignment rate improved to 89.3% after a single mismatch was allowed. Parentage analysis when only four microsatellites were used (a locus with a high frequency of null alleles was excluded) increased the assignment rate to 68% for a perfect mismatch and to 90% for assignment with a single mismatch. Results here confirm that a DNA markerbased approach to parentage assignment in P. hypophthalmus will be useful in genetic improvement programs for this important aquaculture species in Vietnam. .
dc.format.extent 9 pages
dc.publisher The Israeli Journal of Aquaculture - Bamidgeh
dc.subject parentage assignment
dc.subject Pangasianodon hypophthalmus
dc.subject microsatellite
dc.subject multiplex PCR
dc.title Parentage Assignment in Striped Catfish (Pangasianodon hypophthalmus) Based on Microsatellite Marke
dc.type Article
dc.type.dcmi Text
Appears in Collections: Volume 71, 2019

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