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Molecular Cloning, mRNA Expression, and Nutritional Regulation of a Fatty Acyl Δ6-Desaturase-like Gene of the Manchurian Trout, Brachymystax lenok (Pallas)

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Title:Molecular Cloning, mRNA Expression, and Nutritional Regulation of a Fatty Acyl Δ6-Desaturase-like Gene of the Manchurian Trout, Brachymystax lenok (Pallas)
Authors:Jian-hua Y
Shu-guo L
Hua-xin N
Jie C
Zong-fu H
show 1 moreYing H
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Keywords:Brachymystax lenok$fatty acyl delta-6 desaturase-like$cloning$fish oil$sunflower oil$linseed oil
Date Issued:2019
Abstract:The fatty acyl delta-6 desaturase (Δ6-desaturase) is a key rate-limiting
enzyme in the biosynthesis of long-chain polyunsaturated fatty acids (LCPUFAs).
To study the effects of different oil sources in the feed on the
nutritional regulation of Δ6-desaturase-like gene mRNA expression, the fulllength
cDNA of the Δ6-desaturase-like gene was cloned from the liver of
Manchurian trout (Brachymystax lenok). A 9-week feeding trial was
performed, and the fish received diets with three different oil sources: fish oil
(FO), sunflower oil (SO), and linseed oil (LO). The results showed that the
2448 bp long full-length cDNA contained an open reading frame (with a length
of 1365 bp), encoding 454 amino acids. Gene expression analysis indicated
that Δ6-desaturase-like gene mRNA is widely distributed throughout different
tissues, with highest expression levels in both the liver and the brain. The
linolenic acid (ALA) and linoleic acid (LA) compositions were highest in LO and
SO diets, respectively. The eicosapentaenoic acid and docosahexaenoioc acid
composition in LO and SO diets were significantly lower than in the FO diet.
Fish fed with SO and LO showed significantly higher liver expression levels of
Δ6-desaturase-like gene mRNA than those fed with FO. This indicates that SO
and LO in the diet affected the nutritional regulation of the Δ6-desaturase-like
gene mRNA in the liver of the Manchurian trout. In addition, these data
suggest that the Manchurian trout has the ability to synthesize long-chain
unsaturated fatty acids (LC-PUFAs) from ALA and LA.
Pages/Duration:12 pages
URI:http://hdl.handle.net/10524/63205
ISSN:0792-156X
Appears in Collections: Volume 71, 2019


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