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The secondary bacterial infection caused by WSSV outbreaks impacts shrimp Marsupenaeus japonicus’ growth as well as its intestinal microbiota’s composition and function

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Title:The secondary bacterial infection caused by WSSV outbreaks impacts shrimp Marsupenaeus japonicus’ growth as well as its intestinal microbiota’s composition and function
Authors:He, Zihao
Zhao, Jichen
Liao, Xuzheng
Chen, Xieyan
Fu, Zhibin
show 2 moreSun, Chengbo
Ni, Zuotao
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Keywords:marsupenaeus japonicus
wssv
intestinal microbiota
growth performance
secondary bacterial infections
show 5 moreimmunity strategy
shrimp
metagenomics
diagnostics
bacteria
show less
Date Issued:2020
Abstract:Intestinal microbiota homeostasis is a complex ecosystem and is essential for promoting aquatic animals’ growth. Invading pathogens can cause dynamical composition and structure change in the hosts’ microbiota due to this affecting their functions. WSSV is one of the most common and dangerous shrimp pathogens, which leads to white spot disease with too high mortality. A few studies on shrimp intestinal microbiota mainly focused on the changes during acute infection stage. Marsupenaeus japonicus is one of the most cultured shrimp with a decent ability to cope with environmental changes. In the current research, using M. japonicus as a model, we compared the differences of intestinal microbiota between healthy shrimp and the shrimp that survived through the WSSV outbreak. Our study showed that compared to the healthy M. japonicus, the WSSV outbreak disrupted intestinal microbiota structure in the survived shrimp: There was less potential beneficial bacteria and more harmful bacteria. Furthermore, the diversity and total abundance of intestinal microbiota in WSSV-survived shrimp increased significantly. On top of that, a metagenomic analysis by PICRUSt suggested that the changed intestinal microbiota could help the host to combat the secondary bacterial infections caused by WSSV outbreaks by regulating cell growth and death, reducing cell motility, improving energy metabolism, and increasing intestinal enzyme activities regardless the smaller sizes of WSSV-survived shrimp. This could be a result of excessive energy consumption and reduced nutrient-absorbing intestinal microbiota. Our study indicated that shrimp intestinal microbiota plays a fundamental role in combating against secondary infections and regulating the hosts’ growth post WSSV outbreaks. The eventual goal is to development of more efficient diagnostics and therapeutic strategies.
Pages/Duration:17 pages
URI:http://hdl.handle.net/10524/63813
ISSN:0792-156X
DOI:https://doi.org/10.46989/001c.21687
Volume:72
Appears in Collections: Volume 72, 2020


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