Please use this identifier to cite or link to this item: http://hdl.handle.net/10524/63898

Analysis of gut microbiota and immune-related genes during sea cucumber (Apostichopus japonicus) response to dietary supplementation with Codonopsis pilosula

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Title:Analysis of gut microbiota and immune-related genes during sea cucumber (Apostichopus japonicus) response to dietary supplementation with Codonopsis pilosula
Authors:Wang, Xiaolu
Fan, Ying
Yu, Xiaoqing
Wang, Youhong
Ye, Haibin
show 7 moreDiao, Jing
Wang, Shuxian
Liu, Hongjun
Xu, La
Ma, Danping
Gai, Chunlei
Zhang, Tao
show less
Keywords:codonopsis pilosula
apostichopus japonicas
gut microbiota
high-throughput sequencing
immunity
Date Issued:2021
Abstract:The gut microbiota composition of sea cucumber (Apostichopus japonicas) was investigated using high-throughput sequencing techniques. The mRNA expression of complement component 3 and lysozyme genes was evaluated using quantitative fluorescence PCR. Sea cucumbers were fed with a basal diet (control group) and an experimental diet supplemented with Codonopsis pilosula (experimental group) for 30 days. The results showed that the alpha diversity of the gut microbiota was changed in different indices, including Chao1, the abundance-based coverage estimator, the Shannon index, and Good's coverage. Dietary C. pilosula promoted the proliferation of the Flavobacteriaceae family of the Proteobacteria phylum and reduced the relative abundance of the Verrucomicrobiaceae family of the Verrucomicrobia phylum. We concluded that dietary C. pilosula supplementation could alter the network interactions among different microbial functional groups by changing the ecological network's microbial community composition and biological evolution. A positive effect on A. japonicus immune responses in the gut was seen via increasing the mRNA expression of the complement component 3 and lysozyme genes. It seems to happen via modulating the balance in gut microbiota.
Pages/Duration:12 pages
URI:http://hdl.handle.net/10524/63898
ISSN:0792-156X
DOI:https://doi.org/10.46989/001c.25698
Volume:73
Appears in Collections: Volume 73, 2021


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