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Analysis of gut microbiota and immune-related genes during sea cucumber (Apostichopus japonicus) response to dietary supplementation with Codonopsis pilosula
|dc.description.abstract||The gut microbiota composition of sea cucumber (<em>Apostichopus japonicas</em>) was investigated using high-throughput sequencing techniques. The mRNA expression of complement component 3 and lysozyme genes was evaluated using quantitative fluorescence PCR. Sea cucumbers were fed with a basal diet (control group) and an experimental diet supplemented with <em>Codonopsis pilosula</em> (experimental group) for 30 days. The results showed that the alpha diversity of the gut microbiota was changed in different indices, including Chao1, the abundance-based coverage estimator, the Shannon index, and Good's coverage. Dietary <em>C. pilosula</em> promoted the proliferation of the Flavobacteriaceae family of the Proteobacteria phylum and reduced the relative abundance of the Verrucomicrobiaceae family of the Verrucomicrobia phylum. We concluded that dietary <em>C. pilosula</em> supplementation could alter the network interactions among different microbial functional groups by changing the ecological network's microbial community composition and biological evolution. A positive effect on A. japonicus immune responses in the gut was seen via increasing the mRNA expression of the complement component 3 and lysozyme genes. It seems to happen via modulating the balance in gut microbiota.|
|dc.relation.ispartof||The Israeli Journal of Aquaculture - Bamidgeh|
|dc.title||Analysis of gut microbiota and immune-related genes during sea cucumber (<em>Apostichopus japonicus</em>) response to dietary supplementation with <em>Codonopsis pilosula</em>|
|Appears in Collections:||
Volume 73, 2021|
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