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Analysis of gut microbiota and immune-related genes during sea cucumber (Apostichopus japonicus) response to dietary supplementation with Codonopsis pilosula

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Item Summary Wang, Xiaolu Fan, Ying Yu, Xiaoqing Wang, Youhong Ye, Haibin Diao, Jing Wang, Shuxian Liu, Hongjun Xu, La Ma, Danping Gai, Chunlei Zhang, Tao 2021-07-19T19:35:04Z 2021-07-19T19:35:04Z 2021
dc.identifier.issn 0792-156X
dc.description.abstract The gut microbiota composition of sea cucumber (<em>Apostichopus japonicas</em>) was investigated using high-throughput sequencing techniques. The mRNA expression of complement component 3 and lysozyme genes was evaluated using quantitative fluorescence PCR. Sea cucumbers were fed with a basal diet (control group) and an experimental diet supplemented with <em>Codonopsis pilosula</em> (experimental group) for 30 days. The results showed that the alpha diversity of the gut microbiota was changed in different indices, including Chao1, the abundance-based coverage estimator, the Shannon index, and Good's coverage. Dietary <em>C. pilosula</em> promoted the proliferation of the Flavobacteriaceae family of the Proteobacteria phylum and reduced the relative abundance of the Verrucomicrobiaceae family of the Verrucomicrobia phylum. We concluded that dietary <em>C. pilosula</em> supplementation could alter the network interactions among different microbial functional groups by changing the ecological network's microbial community composition and biological evolution. A positive effect on A. japonicus immune responses in the gut was seen via increasing the mRNA expression of the complement component 3 and lysozyme genes. It seems to happen via modulating the balance in gut microbiota.
dc.format.extent 12 pages
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject codonopsis pilosula
dc.subject apostichopus japonicas
dc.subject gut microbiota
dc.subject high-throughput sequencing
dc.subject immunity
dc.title Analysis of gut microbiota and immune-related genes during sea cucumber (<em>Apostichopus japonicus</em>) response to dietary supplementation with <em>Codonopsis pilosula</em>
dc.type Article
dc.type.dcmi Text
prism.volume 73
Appears in Collections: Volume 73, 2021

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