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Analysis of gut microbiota and immune-related genes during sea cucumber (Apostichopus japonicus) response to dietary supplementation with Codonopsis pilosula

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dc.contributor.author Wang, Xiaolu
dc.contributor.author Fan, Ying
dc.contributor.author Yu, Xiaoqing
dc.contributor.author Wang, Youhong
dc.contributor.author Ye, Haibin
dc.contributor.author Diao, Jing
dc.contributor.author Wang, Shuxian
dc.contributor.author Liu, Hongjun
dc.contributor.author Xu, La
dc.contributor.author Ma, Danping
dc.contributor.author Gai, Chunlei
dc.contributor.author Zhang, Tao
dc.date.accessioned 2021-07-19T19:35:04Z
dc.date.available 2021-07-19T19:35:04Z
dc.date.issued 2021
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/63898
dc.description.abstract The gut microbiota composition of sea cucumber (<em>Apostichopus japonicas</em>) was investigated using high-throughput sequencing techniques. The mRNA expression of complement component 3 and lysozyme genes was evaluated using quantitative fluorescence PCR. Sea cucumbers were fed with a basal diet (control group) and an experimental diet supplemented with <em>Codonopsis pilosula</em> (experimental group) for 30 days. The results showed that the alpha diversity of the gut microbiota was changed in different indices, including Chao1, the abundance-based coverage estimator, the Shannon index, and Good's coverage. Dietary <em>C. pilosula</em> promoted the proliferation of the Flavobacteriaceae family of the Proteobacteria phylum and reduced the relative abundance of the Verrucomicrobiaceae family of the Verrucomicrobia phylum. We concluded that dietary <em>C. pilosula</em> supplementation could alter the network interactions among different microbial functional groups by changing the ecological network's microbial community composition and biological evolution. A positive effect on A. japonicus immune responses in the gut was seen via increasing the mRNA expression of the complement component 3 and lysozyme genes. It seems to happen via modulating the balance in gut microbiota.
dc.format.extent 12 pages
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject codonopsis pilosula
dc.subject apostichopus japonicas
dc.subject gut microbiota
dc.subject high-throughput sequencing
dc.subject immunity
dc.title Analysis of gut microbiota and immune-related genes during sea cucumber (<em>Apostichopus japonicus</em>) response to dietary supplementation with <em>Codonopsis pilosula</em>
dc.type Article
dc.type.dcmi Text
prism.volume 73
dc.identifier.doi https://doi.org/10.46989/001c.25698
Appears in Collections: Volume 73, 2021


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