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Transcriptome analysis of Marsupenaeus japonicus hepatopancreas during WSSV persistent infection

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dc.contributor.author Liao, Minze
dc.contributor.author Zhao, Jichen
dc.contributor.author He, Zihao
dc.contributor.author Chen, Xieyan
dc.contributor.author Xue, Yuan
dc.contributor.author Zhou, Jianing
dc.contributor.author Long, Xinxin
dc.contributor.author Sun, Chengbo
dc.date.accessioned 2021-08-23T20:08:00Z
dc.date.available 2021-08-23T20:08:00Z
dc.date.issued 2021
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/63909
dc.description.abstract White Spot Syndrome Virus (WSSV) can cause a large-scale death of cultured shrimp and significant damage to the shrimp farming industry. Marsupenaeus japonicus is one of the world's most important economically farmed shrimp. This study found that some <em>M. japonicus</em> survived the spontaneous outbreak of WSSV. Surprisingly, these virus-carrying shrimp showed no apparent illnesses or outbreaks of white spot disease in the subsequent cultivation, and their body size was substantially smaller than healthy shrimp, indicating a long-term fight between the host and the virus. To investigate this interesting phenomenon, we analyzed the transcriptomes of healthy shrimp and survived shrimp through the RNA-Seq platform, attempting to reveal the underlying molecular mechanism of the struggle between M. japonicus and WSSV. Transcriptional analysis showed that a total of 37,815 unigenes were assembled, with an average length of 1,193.34 bp and N50 of 2,049 bp. In the KEGG pathway, enrichment analysis of DEGs pathways related to immunity, biosynthesis, and growth metabolism was enriched, including pentose phosphate pathway, glycerophospholipid metabolism, fatty acid biosynthesis, Wnt signaling pathway, biosynthesis of amino acids, ascorbate, and aldarate metabolism. Our data showed a delicate balance between <em>M. japonicus</em> and WSSV infection: On the one hand, WSSV infection can cause host metabolism and biosynthesis disorders in the host, and the virus consumes a portion of the material and energy required for shrimp average growth and reproduction. If WSSV infection persisted for a long time, then the growth rate of <em>M. japonicus</em> decreased. On the other hand, the host can regulate immune defense to resist subsequent viral infection. This study reveals the underlying molecular mechanism of a long-term battle of M. japonicus against WSSV infection, providing novel insights for preventing WSSV persistent infection in <em>M. japonicus</em> and other farmed shrimp species.
dc.format.extent 15 pages
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject white spot syndrome virus
dc.subject marsupenaeus japonicus
dc.subject persistent infection
dc.subject transcriptome analysis
dc.subject immunity strategy
dc.title Transcriptome analysis of <em>Marsupenaeus japonicus</em> hepatopancreas during WSSV persistent infection
dc.type Article
dc.type.dcmi Text
prism.volume 73
dc.identifier.doi https://doi.org/10.46989/001c.27662
Appears in Collections: Volume 73, 2021


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