Please use this identifier to cite or link to this item:

Expression analysis of three immune genes IL-8, IL-6 and IL-1β in the Japanese flounder (Paralichthys olivaceus)

File Size Format  
31194.pdf 862.55 kB Adobe PDF View/Open

Item Summary Pan, Qiuli Zhang, Feng Liu, Yang Zang, Lin Wang, Xiuli Qiu, Xuemei 2021-12-28T18:44:29Z 2021-12-28T18:44:29Z 2021
dc.identifier.issn 0792-156X
dc.description.abstract Interleukin-8 (IL-8), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β) are major immune-related genes that play important roles in the innate immune system of vertebrates. In this study, the expression levels of the three immune-related genes in eleven tissues of normal adult Japanese flounder (Paralichthys olivaceus) were examined using semi-quantitative RT-PCR at 13time points after challenge with Lipopolysaccharide (LPS). The results showed that the highest expression levels of the Japanese flounder IL-8 were detected at 12h in the spleen, 12h in the head kidney, and 24h in the liver after the challenge with LPS. Interestingly, the expression levels of IL-6 in the spleen and head kidney were highest at 48h. The liver had the highest expression level of IL-6 at 36h.The highest expression levels of IL-1β were detected at 3h in the spleen and 9h in the head kidney and liver. These results indicated that IL-8, IL-6, and IL-1β also played important roles in the immune response of the Japanese flounder at lower temperatures. The study provided a basis for further research on the immune mechanisms of IL-8, IL-6, IL-1β, and the production of recombinant IL-8, IL-6, or IL-1β used in Japanese flounder cultivation.
dc.format.extent 12 pages
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject Paralichthys olivaceus
dc.subject IL-8
dc.subject IL-6
dc.subject IL-1β
dc.subject LPS
dc.subject tissue expression
dc.subject Japanese flounder
dc.subject immune genes
dc.title Expression analysis of three immune genes IL-8, IL-6 and IL-1β in the Japanese flounder (Paralichthys olivaceus)
dc.type Article
dc.type.dcmi Text
prism.volume 73
Appears in Collections: Volume 73, 2021

Please email if you need this content in ADA-compliant format.

Items in eVols are protected by copyright, with all rights reserved, unless otherwise indicated.