Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon

Date
2022
Authors
Li, Yun-Dong
Zhou, Fa-Lin
Yang, Qi-Bin
Jiang, Song
Yang, Li-Shi
Huang, Jian-Hua
Chen, Xu
Mai, Qianlin
Jiang, Shi-Gui
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74
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Abstract
A novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%~91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was highest expressed in the hepatopancreas and the lowest expressed in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon.
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Keywords
Gene clone, Growth traits-related, Penaeus monodon, Single nucleotide polymorphism (SNP), Trypsin gene (PmTry)
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15 pages
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Related To
The Israeli Journal of Aquaculture - Bamidgeh
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