Establishment of a Multiplex PCR Assay to Detect Five Major Freshwater Bacteria

dc.contributor.author Gao, Weihua
dc.contributor.author Ai, Kete
dc.contributor.author Luo, Kai
dc.contributor.author Huang, Tinghua
dc.contributor.author Yao, Min
dc.contributor.author Hu, Wei
dc.contributor.author Fang, Liu
dc.contributor.author Qi, Zhitao
dc.contributor.author Xu, Qiaoqing
dc.date.accessioned 2017-12-28T02:32:00Z
dc.date.available 2017-12-28T02:32:00Z
dc.date.issued 2017
dc.description.abstract A multiplex polymerase chain reaction (mPCR) method for simultaneous detection of Aeromonas hydrophila, Streptococcus agalactiae, Klebsiella pneumoniae, Edwardsiella tarda, and E. ictalur was developed to rapidly and accurately identify the five most common bacteria that infect aquatic animals. The expected amplicons for ahe2 gene of A. hydrophila, cpsE gene of S. agalactiae, khe gene of K. pneumoniae, mukF gene of E.tarda, and the serC gene of E. ictaluri were 853 bp, 685 bp, 428 bp, 356 bp, and 124 bp, respectively. In the single PCR assays, the minimum detectable DNA contents were 13.2 pg for A. hydrophila, 27.4 pg for S. agalactiae, 1.95 pg for K. pneumoniae, 1.63 pg for E. tarda, 1.02 pg for E. ictalur. The detection limits of the multiplex PCR were 0.66 ng, 1.91 ng, 0.68 ng, 0.41 ng, 0.71 ng for A. hydrophila, S. agalactiae, K. pneumoniae, E. tarda and E. ictalur, respectively. The established multiplex PCR is significant for the rapid detection of common pathogenic bacteria of aquatic animals and provides the basis for the diagnosis of fish diseases.
dc.format.extent 9 pages
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/57034
dc.subject multiplex PCR
dc.subject freshwater bacteria
dc.subject detection
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture
dc.title Establishment of a Multiplex PCR Assay to Detect Five Major Freshwater Bacteria
dc.type Article
dc.type.dcmi Text
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