Establishment of a Multiplex PCR Assay to Detect Five Major Freshwater Bacteria

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dc.contributor.authorGao, Weihua
dc.contributor.authorAi, Kete
dc.contributor.authorLuo, Kai
dc.contributor.authorHuang, Tinghua
dc.contributor.authorYao, Min
dc.contributor.authorHu, Wei
dc.contributor.authorFang, Liu
dc.contributor.authorQi, Zhitao
dc.contributor.authorXu, Qiaoqing
dc.date.accessioned2017-12-28T02:32:00Z
dc.date.available2017-12-28T02:32:00Z
dc.date.issued2017
dc.description.abstractA multiplex polymerase chain reaction (mPCR) method for simultaneous detection of Aeromonas hydrophila, Streptococcus agalactiae, Klebsiella pneumoniae, Edwardsiella tarda, and E. ictalur was developed to rapidly and accurately identify the five most common bacteria that infect aquatic animals. The expected amplicons for ahe2 gene of A. hydrophila, cpsE gene of S. agalactiae, khe gene of K. pneumoniae, mukF gene of E.tarda, and the serC gene of E. ictaluri were 853 bp, 685 bp, 428 bp, 356 bp, and 124 bp, respectively. In the single PCR assays, the minimum detectable DNA contents were 13.2 pg for A. hydrophila, 27.4 pg for S. agalactiae, 1.95 pg for K. pneumoniae, 1.63 pg for E. tarda, 1.02 pg for E. ictalur. The detection limits of the multiplex PCR were 0.66 ng, 1.91 ng, 0.68 ng, 0.41 ng, 0.71 ng for A. hydrophila, S. agalactiae, K. pneumoniae, E. tarda and E. ictalur, respectively. The established multiplex PCR is significant for the rapid detection of common pathogenic bacteria of aquatic animals and provides the basis for the diagnosis of fish diseases.
dc.format.extent9 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/57034
dc.subjectmultiplex PCR
dc.subjectfreshwater bacteria
dc.subjectdetection
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture
dc.titleEstablishment of a Multiplex PCR Assay to Detect Five Major Freshwater Bacteria
dc.typeArticle
dc.type.dcmiText

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Volume 69, 2017