Effect of dietary glycerol monolaurate supplementation on gene expression, intestinal mucosa and microbiota of juvenile black sea bream (<em>Acanthopagrus schlegelii</em>).

Abstract

Glycerol monolaurate (GML) is a chemical compound derived from the combination of lauric acid and glycerol, exhibiting potent antimicrobial properties. An eight-week feeding trial was conducted to evaluate the effects of dietary supplementation of Glycerol Monolaurate (GML) on juvenile black sea bream. The control diet included 24% fish meal, while five additional diets were formulated with increasing GML concentrations: 0.01% (GML1), 0.02% (GML2), 0.04% (GML3), 0.08% (GML4), and 0.16% (GML5). Each diet was assigned to triplicate tanks. At the end of the experiment, serum immune and antioxidant in liver were non-significantly different among the groups. The relative gene expression of IGF-1 and GH in liver was statistically higher in the GML3 treated. The relative gene expression of IL-1, TGF, TNF-1, CCK, CLDN1, CASS-3 and GPR41 in intestine were statistically higher in the GML3 group. The photomicrographs of transmission electron microscope of the intestine were also studied. The GML supplemented groups had longer brush borders. The cells seemed to be joined by an intact junctional complex without visible intercellular spaces. The observed variations in phyla, class, and genus did not demonstrate statistical significance. Proteobacteria, Cyanobacteria, Firmicutes and Actinobacteria in the phylum, and Oxyphotobacteria, Bacteroidia, Holophagae and Negativicutes in the class, <em>Brevundimonas</em>, and <em>Achromobacter</em> in the genera were all substantially increased with higher levels of GML supplementation. Based on these results, it is suggested that glycerol monolaurate (GML) may serve as a potential dietary supplement for promoting different gene expression and intestinal health in black sea bream.