Identification and mRNA Expression of Antioxidant Enzyme Genes in the Mud Crab (Scylla paramamosain) in Response to Acute Ammonia and Nitrite Exposure

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dc.contributor.authorChenga, C-H
dc.contributor.authorMaa, H.-L.
dc.contributor.authorFenga, J.
dc.contributor.authorSua, Y-L.
dc.contributor.authorDenga, Y-Q.
dc.contributor.authorPenga, J-H.
dc.contributor.authorGuo, Z-X
dc.date.accessioned2018-05-11T19:58:00Z
dc.date.available2018-05-11T19:58:00Z
dc.date.issued2018
dc.description.abstractThioredoxin reductase (TrxR) is a conserved protein that is involved in protecting organisms against various oxidative stresses. In this study, a thioredoxin reductase gene was cloned from the mud crab Scylla paramamosain (SpTrxR). The full-length cDNA of SpTrxR is comprised of 2724 bp with a 1791 bp open reading frame that encodes a putative protein of 596 amino acids. The deduced amino acid sequence of SpTrxR contains the typical TrxR domain. Quantitative real-time PCR analysis revealed that the SpTrxR mRNA was distributed abundantly in mud crabs, while strong expression was observed mainly in the gills. The expression of antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) was measured using quantitative real-time PCR after acute ammonia and nitrite exposure. The results show that antioxidant enzyme genes (SpTrxR, SpTrx, SpSOD, and SpCAT) were modulated by acute ammonia and nitrite exposure. These results suggest that antioxidant enzyme genes play an important role in protecting organisms against oxidative stress
dc.format.extent13 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/57165
dc.subjectScylla paramamosain
dc.subjectantioxidant enzyme genes
dc.subjectthioredoxin reductase
dc.subjectambient stressors
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture
dc.titleIdentification and mRNA Expression of Antioxidant Enzyme Genes in the Mud Crab (Scylla paramamosain) in Response to Acute Ammonia and Nitrite Exposure
dc.typeArticle
dc.type.dcmiText

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Volume 70, 2018