Epidemiological Survey of Grass Carp (Ctenopharyngodon idella) Reovirus in South China, and Genetic Variations of VP6 gene

dc.contributor.author Yang, Ying
dc.contributor.author Peng, Zhongqin
dc.contributor.author Li, Hua
dc.contributor.author Tan, Shuwen
dc.contributor.author Yu, Haiyi
dc.contributor.author Yu, Hui
dc.date.accessioned 2017-12-28T02:31:31Z
dc.date.available 2017-12-28T02:31:31Z
dc.date.issued 2017
dc.description.abstract To study the molecular biology characteristics, phylogenetic relationship, and status quo of grass carp reovirus (GCRV) prevalent isolates, we conducted an epidemiological survey in South China from 2012 to 2016. We sampled 126 fish collected from five provinces. Eight strains of GCRV were isolated and examined in order to understand their genetic characteristics and evolution regularity. The results showed three genotypes of GCRV, of which. Genotype Ⅱ was found to be the prevalent strain in South China. SDS-PAGE electrophoresis further suggested that all viruses possess 11 segments of dsRNA, which have typical characteristics of GCRV. Based on amino acid sequence phylogenetic tree of VP6 gene, all eight isolates belonged to genotype Ⅱ and had significant variations compared to genotype Ⅰ. Analysis of amino acid sequence showed that these two genotypes of GCRV had no immunological cross-reactions. These results indicated that genotype Ⅱ GCRV is an extensive pandemic, and revealed new genetic diversity in China.
dc.format.extent 9 pages
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/57030
dc.subject Grass carp reovirus
dc.subject virus isolates
dc.subject genetic variation
dc.subject identification
dc.subject immunogenicity
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture
dc.title Epidemiological Survey of Grass Carp (Ctenopharyngodon idella) Reovirus in South China, and Genetic Variations of VP6 gene
dc.type Article
dc.type.dcmi Text
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