Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid

dc.contributor.author Kecheng Zhu
dc.contributor.author Nan Zhang
dc.contributor.author Huayang Guo
dc.contributor.author Shigui Jiang
dc.contributor.author Dianchang Zhang
dc.date.accessioned 2017-01-27T18:52:33Z
dc.date.available 2017-01-27T18:52:33Z
dc.date.issued 2016
dc.description.abstract Myeloid differentiation factor 88 (MyD88) is a key and essential adapter involved in the interleukin-1 receptor (IL-1R) and toll-like receptor (TLR)-mediated activation signaling pathway. To investigate molecular characterization of MyD88 and its gene expression profile in response to stimulation by lipopolysaccharide (LPS) and polyinosinic-cytidylic acid (poly (I: C)), we isolated the MyD88 cDNA sequence in Pinctada fucata and analyzed expression patterns using quantitative real-time PCR. Sequence analysis indicated that Pf-MyD88 cDNA is 1463bp in length and contains a1050bp open reading frame that encodes a 349 α peptide. Pf-MyD88 has the highest similarity with homologues of Crassostrea gigas and highly conserved death and toll/IL-1R domains. Furthermore, during LPS and poly (I:C)-stimulated experiments in the gill, peak expression levels of Pf-MyD88 were detected at 2h and 8h with a 1.58-fold and 3.58-fold increase, respectively. The results revealed the existence of a MyD88-dependent signaling pathway in P. fucata and contributed to understanding the potential role of Pf-MyD88 in the TLR/IL-1R-mediated signaling pathway.
dc.format.extent 9 pages
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/54942
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject Pinctada fucata
dc.subject MyD88
dc.subject LPS and poly (I: C)
dc.subject gene expression
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture.
dc.title Molecular Characterization of MyD88 in Pinctada fucata and its Response to Lipopolysaccharides and Polyinosinic-cytidylic Acid
dc.type Article
dc.type.dcmi Text
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