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Title: Detection of KHV in Freshwater Mussels and Crustaceans from Ponds with KHV History in Common Carp (Cyprinus carpio) 
Author: Kielpinski, Maciej; Kempter, Jolanta; Panicz, Remigiusz; Sadowski, Jacek; Schütze, Heike; Ohlemeyer, Stefanie; Bergmann, Sven M.
Date: 2010
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Kielpinski, M., Kempter, J., Panicz, R., Sadowski, J., Schütze, H., Ohlemeyer, S., & Bergmann, S.M. (2010). Detection of KHV in Freshwater Mussels and Crustaceans from Ponds with KHV History in Common Carp (Cyprinus carpio). The Israeli Journal of Aquaculture - Bamidgeh, 62(1), 28-37.
Abstract: Characterization of asymptomatic KHV carriers may help understand virus transmission and storage. Such information allows farmers to minimize KHV on farms where this virus is present, also in common carp (Cyprinus carpio) monocultures. As asymptomatic KHV carriers, freshwater mollusks from the Unionidae family (swan mussels, Anodonta cygnea) and crustaceans from the Gammaridae family (scud, Gammarus pulex) were studied because of their unique method of feeding by accumulating bacterial and viral particles. The KHV genome was detected by nested PCR and confirmed by PCR recognizing the KHV glycoprotein gene in ORF 56 (KHV-U). Both PCR and nested PCR, which recognize the KHV thymidine kinase gene, always produced negative results in the swan mussels and scud samples.
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 10 pages
ISSN: 0792-156X
URI: http://hdl.handle.net/10524/19307
Keywords: koi herpesvirus detection, freshwater mollusks, PCR
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.
Aquaculture--Israel--Periodicals.
Aquaculture--Periodicals.

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