Transcriptome Analysis of the Cultured Hybrid Grouper (♀*Epinephelus fuscoguttatus*×♂*E. lanceolatus*) Immunized with <em>Vibrio harveyi</em> formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide

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dc.contributor.authorWan, Xiaoju
dc.contributor.authorDa, Fan
dc.contributor.authorLin, Guixiang
dc.contributor.authorHuang, Yucong
dc.contributor.authorCai, Shuanghu
dc.contributor.authorJian, Jichang
dc.date.accessioned2023-11-27T21:01:37Z
dc.date.available2023-11-27T21:01:37Z
dc.date.issued2023
dc.description.abstractGrouper has become an essential mariculture species in China, while vibriosis caused by Vibrio harveyi significantly impacts its culture. Our previous study confirmed the *V. harveyi* formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide (FKC+COS) effectively prevents vibriosis. As an adjuvant, COS could significantly enhance FKC effectiveness against *V. harveyi* in grouper. In the present study, we performed transcriptome analysis of grouper spleens tissue 14 days post-immunization of PBS and FKC+COS, respectively. After assembly and annotation, 2,503 differentially expressed genes (DEGs) were obtained, including the upregulated 1,894 DEGs and downregulated 609 DEGs between the PBS group and FKC+COS group. To explore the relevance of DEGs in immunity, enrichment analysis in the KEGG database revealed that the main pathways of DEGs distribution associated with immunity were antigen processing and presentation, lysosome, the intestinal immune network for IgA production and FcγR-mediated phagocytosis. In conclusion, transcriptome analysis of spleens was performed to explore the potential mechanism of COS as an adjuvant enhancing the protection effectiveness of FKC against vibriosis in grouper.
dc.format.extentnull pages
dc.identifier.doihttps://doi.org/10.46989/001c.88370
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/73296
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectCOS
dc.subjecthybrid grouper
dc.subjectinactivated vaccines
dc.subjectVibrio harveyi
dc.titleTranscriptome Analysis of the Cultured Hybrid Grouper (♀*Epinephelus fuscoguttatus*×♂*E. lanceolatus*) Immunized with <em>Vibrio harveyi</em> formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide
dc.typeArticle
dc.type.dcmiText
dspace.entity.type
prism.volume75

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Volume 75, Issue 2, 2023