Transcriptome Analysis of the Cultured Hybrid Grouper (♀*Epinephelus fuscoguttatus*×♂*E. lanceolatus*) Immunized with Vibrio harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide

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dc.contributor.author Wan, Xiaoju
dc.contributor.author Da, Fan
dc.contributor.author Lin, Guixiang
dc.contributor.author Huang, Yucong
dc.contributor.author Cai, Shuanghu
dc.contributor.author Jian, Jichang
dc.date.accessioned 2023-11-27T21:01:37Z
dc.date.available 2023-11-27T21:01:37Z
dc.date.issued 2023
dc.description.abstract Grouper has become an essential mariculture species in China, while vibriosis caused by Vibrio harveyi significantly impacts its culture. Our previous study confirmed the *V. harveyi* formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide (FKC+COS) effectively prevents vibriosis. As an adjuvant, COS could significantly enhance FKC effectiveness against *V. harveyi* in grouper. In the present study, we performed transcriptome analysis of grouper spleens tissue 14 days post-immunization of PBS and FKC+COS, respectively. After assembly and annotation, 2,503 differentially expressed genes (DEGs) were obtained, including the upregulated 1,894 DEGs and downregulated 609 DEGs between the PBS group and FKC+COS group. To explore the relevance of DEGs in immunity, enrichment analysis in the KEGG database revealed that the main pathways of DEGs distribution associated with immunity were antigen processing and presentation, lysosome, the intestinal immune network for IgA production and FcγR-mediated phagocytosis. In conclusion, transcriptome analysis of spleens was performed to explore the potential mechanism of COS as an adjuvant enhancing the protection effectiveness of FKC against vibriosis in grouper.
dc.format.extent null pages
dc.identifier.doi https://doi.org/10.46989/001c.88370
dc.identifier.issn 0792-156X
dc.identifier.uri https://hdl.handle.net/10524/73296
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject COS
dc.subject hybrid grouper
dc.subject inactivated vaccines
dc.subject Vibrio harveyi
dc.title Transcriptome Analysis of the Cultured Hybrid Grouper (♀*Epinephelus fuscoguttatus*×♂*E. lanceolatus*) Immunized with <em>Vibrio harveyi</em> formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide
dc.type Article
dc.type.dcmi Text
dspace.entity.type
prism.volume 75
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Volume 75, Issue 2, 2023