Efficacy of a formalin-inactivated vaccine against <em>Lactococcus garvieae</em> infection in golden pompano <em>Trachinotus ovatus</em>
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2023
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75
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*Lactococcus garvieae* is one of the primary pathogens causing the severe disease to golden pompano (*Trachinotus ovatus*). Vaccination is the most effective measure of preventing the occurrence and spread of infectious diseases. However, no commercially available vaccine protects *T. ovatus* against *L. garvieae* infection. In the present study, the formalin-inactivated *L. garvieae* vaccines, consisting of bacterin alone (aqueous vaccine) and combined with oil adjuvant Montanide™ ISA 763 A VG (vaccine+763A), were prepared and administered to *T. ovatus* by intraperitoneal injection. The protective efficacy was evaluated by testing the relative percent survival (RPS), serum enzyme activity, and expression levels of immune-related genes in the tissues of *T. ovatus* after immunization. The results indicated that the relative percentage survival (RPS) values of aqueous vaccine and vaccine+763A were 80% and 90.8% at 8 weeks post-vaccination. The level of antibody titer, lysozyme activities, superoxide dismutase, and catalase in the two vaccinated fish groups increased significantly compared to that in the control group. Moreover, the expression levels of immune-related genes *IgM*, *TNF-α*, *IL-8*, *MHCI-α*, *MHCII*, *CD4*, *CD8α,* and *IL-1β* in all the tested tissues from *T. ovatus* were significantly enhanced after inoculation with aqueous vaccine and vaccine+763A. These findings demonstrated that the developed vaccine combined with oil adjuvant ISA 763 A VG improved immune responses and provided good protective efficacy against *L. garvieae* infection in the golden pompano culture.
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Lactococcus garvieae, Trachinotus ovatus, oil adjuvant, inactivated vaccine, golden pompano
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null pages
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The Israeli Journal of Aquaculture - Bamidgeh
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