Use Of Glucan From Saccharomyces Cerevisiae As An Immunostimulant In Carp: Impact On Hematology, Phagocyte Function, And Infection With Aeromonas Hydrophila

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dc.contributor.authorSelvaraj, V.
dc.contributor.authorSampath, K.
dc.contributor.authorSekar, V.
dc.date.accessioned2012-01-19T20:14:13Z
dc.date.available2012-01-19T20:14:13Z
dc.date.issued2005
dc.description.abstractGlucan was extracted from yeast (Saccharomyces cerevisiae) and subjected to chemical analy- ses. Thin layer chromatography (TLC) revealed that glucan is primarily composed of glucose units and contains 97.2% glucose. Infrared spectrum showed a major band at 883/cm, suggest- ing that glucan is linked to β-glycosidic. Intraperitoneal administration of different doses of glu- can enhanced the survival rate of carp (Cyprinus carpio) infected by Aeromonas hydrophila. When a concentration above 500 μg glucan per fish was administered, survival relative to the uninfected control was 100%. The total leukocyte count and neutrophil and monocyte popula- tions increased after administration of the glucan. The maximum effect occurred on day 6 after injection. Macrophages of compound-administrated test animals significantly increased produc- tion of superoxide anions, efficiently killing the bacterial pathogen. This study showed that glu- can can be effective in inducing non-specific cellular defense mechanism in carp.
dc.format.extent10 pages
dc.identifier.citationSelvaraj, V., Sampath, K., & Sekar, V. (2005). Use Of Glucan From Saccharomyces Cerevisiae As An Immunostimulant In Carp: Impact On Hematology, Phagocyte Function, And Infection With Aeromonas Hydrophila. The Israeli Journal of Aquaculture - Bamidgeh, 57(1), 39-48.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19128
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectcarp, leukocytes, macrophage, monocytes, NBT assay, neutrophils, opsonin, phagocytes
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleUse Of Glucan From Saccharomyces Cerevisiae As An Immunostimulant In Carp: Impact On Hematology, Phagocyte Function, And Infection With Aeromonas Hydrophila

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IJA Volume 57, Issue 1, 2005