Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata

Simple item page
dc.contributor.author Liu, Yun-Guo
dc.contributor.author Chen, Song-Lin
dc.contributor.author Liu, Chun-Ying
dc.contributor.author Li, Fang-Zheng
dc.date.accessioned 2012-01-19T20:23:01Z
dc.date.available 2012-01-19T20:23:01Z
dc.date.issued 2008
dc.description.abstract A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreas- ing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.
dc.format.extent 7 pages
dc.identifier.citation Liu, Y., Chen, S., Liu, C., & Li, F. (2008). Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 75-81.
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/19249
dc.publisher Israeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseries The Israeli Journal of Aquaculture - Bamidgeh
dc.subject red sea bream, gilthead sea bream, EST, SSR, microsatellite
dc.subject.lcsh Fish culture--Israel--Periodicals.
dc.subject.lcsh Fish culture--Periodicals.
dc.subject.lcsh Aquaculture--Israel--Periodicals.
dc.subject.lcsh Aquaculture--Periodicals.
dc.title Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
60_2_Liu.pdf
Size:
314.84 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
IJA Volume 60, Issue 2, 2008