Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata

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dc.contributor.authorLiu, Yun-Guo
dc.contributor.authorChen, Song-Lin
dc.contributor.authorLiu, Chun-Ying
dc.contributor.authorLi, Fang-Zheng
dc.date.accessioned2012-01-19T20:23:01Z
dc.date.available2012-01-19T20:23:01Z
dc.date.issued2008
dc.description.abstractA total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreas- ing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.
dc.format.extent7 pages
dc.identifier.citationLiu, Y., Chen, S., Liu, C., & Li, F. (2008). Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 75-81.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19249
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectred sea bream, gilthead sea bream, EST, SSR, microsatellite
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleAnalysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata

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IJA Volume 60, Issue 2, 2008