Screening of sex-special genes in common long-arm octopus <em>Octopus minor</em> based on gonadal transcriptome sequencing

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dc.contributor.authorHe, Jiabei
dc.contributor.authorWang, He
dc.contributor.authorLiu, Chao
dc.contributor.authorChen, Siqing
dc.contributor.authorZhao, Qiang
dc.contributor.authorGe, Jianlong
dc.contributor.authorLiu, Yongsheng
dc.date.accessioned2024-09-12T19:50:44Z
dc.date.available2024-09-12T19:50:44Z
dc.date.issued2024
dc.description.abstractThe common long-arm octopus (<em>Octopus minor</em>) is a commercially important aquaculture species in East Asia, and the male octopus grows faster than the female ones, while the information about sex-regulating mechanisms in octopuses is limited. Therefore, gonadal transcriptome sequencing was performed in <em>O. minor</em> to reveal the molecular mechanisms of sex regulation in cephalopods. Based on the sexuality and gonad development stage, 4 groups of 12 gonad tissues were sampled, and 11 libraries were retained for bioinformatics analysis. A total of 263,749,727 clean reads were obtained. The percentage of clean reads mapped to the <em>O. minor</em> reference genome ranged from 77.20% to 90.59%. A total of 3936 differentially expressed genes (DEGs) were obtained between ovarian and testicular libraries by Venn diagram analysis, including 855 ovarian up-regulated and 3081 testicular up-regulated genes. Four unigenes (transcription factor Sox-8, fermentation-1, forkhead box protein L2, and ribosomal protein large 24) and one pathway (ECM-receptor interaction) were screened to be candidate molecular markers for sex determination. The reliability and accuracy of our analysis were validated via quantitative real-time PCR in 10 randomly selected DEGs. The results of our study enhanced our understanding of the molecular mechanism underlying sex determination and potentially helped to screen the bio-markers for <em>O. minor</em> in different sexes.
dc.identifier.doihttps://doi.org/10.46989/001c.123000
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/77344
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectCephalopod
dc.subjectOctopus minor
dc.subjectTranscriptome
dc.subjectSex differentiation
dc.subjectRNA-Seq
dc.titleScreening of sex-special genes in common long-arm octopus <em>Octopus minor</em> based on gonadal transcriptome sequencing
dc.typeArticle
dc.type.dcmiText
prism.volume76

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Volume 76, Issue 3, 2024