Vitrification of Common Carp (Cyprinus Carpio) Spermatozoa, Post-Thaw Sperm Quality, and Fertility

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dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYıldız, Cengiz
dc.contributor.authorYavaş, İlker
dc.date.accessioned2016-04-19T21:22:50Z
dc.date.available2016-04-19T21:22:50Z
dc.date.issued2014
dc.description.abstractThe aim of this investigation was to test a new technology, vitrification, or ultra-rapid freezing of the spermatozoa of common carp, and to study the ability of glucose, BSA, and other cryoprotectants to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified using 10 different cryoprotectant solutions: (1) Glucose based medium (GBM) + 1% bovine serum albumin (BSA); (2) GBM + 1% BSA + 10% DMSO; (3) GBM + 1% BSA + 20% DMSO; (4) GBM + 1% BSA + 30% DMSO; (5) GBM + 1% BSA + 10% DMA; (6) GBM + 1% BSA + 20% DMA; (7) GBM + 1% BSA + 30% DMA; (8) GBM + 1% BSA + 10% methanol; (9) GBM + 1% BSA + 20% methanol; (10) GBM + 1% BSA + 30% methanol. Fertilization rates for vitrification experiments were low and the use of low concentrations of cryoprotectants yielded lower fertilization rates than the vitrification solutions containing high cryoprotectant concentrations. In conclusion, this study reported successful vitrification of common carp spermatozoa by direct transfer into liquid nitrogen.
dc.format.extent7 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/49138
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectvitrification
dc.subjectcryoprotectants
dc.subjectsperm quality
dc.subjectfertilization
dc.subjectCyprinus carpio
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture.
dc.titleVitrification of Common Carp (Cyprinus Carpio) Spermatozoa, Post-Thaw Sperm Quality, and Fertility
dc.typeArticle
dc.type.dcmiText

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Volume 66, 2014