Molecular Characterization and Possible Immune Function of Two Members of Interleukin Family from Trachinotus ovatus

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dc.contributor.authorZhu, Kecheng
dc.contributor.authorGuo, Huayang
dc.contributor.authorZhang, Nan
dc.contributor.authorJiang, Shigui
dc.contributor.authorZhang, Dianchang
dc.date.accessioned2017-12-28T02:33:14Z
dc.date.available2017-12-28T02:33:14Z
dc.date.issued2017
dc.description.abstractInterleukins (ILs) are a group of cytokines which play a core regulatory role in the immune system. In the present study, two members of the IL family, IL7 and IL8, were detected in Trachinotus ovatus. IL7 and IL8 cDNAs of T. ovatus consist of a 492 bp and 300 bp ORF (open reading frame) encoding a polypeptide of 163 and 99 amino acids, respectively. Multiple sequence alignment revealed that IL7 and IL8 contain characteristic arrangements of several conserved cysteine residues, which in T. ovatus are in positions 20, 57, 67, 105, 140, 152 and 35, 37, 61, 78, respectively. The phylogenetic tree showed that all ILs fell into four categories. Moreover, IL7 and IL8 mRNA of T. ovatus were constitutively expressed at different levels in all examined tissues, except muscle. Transcripts of IL7 were mainly expressed in liver, intestine, kidney, stomach, and fin, while transcripts of IL8 were highly detected in the eye, liver, kidney, and intestine of healthy fish. After Photobacterium damselae innoculation, mRNA levels of IL7 were higher than IL8 in the spleen and intestine, however, mRNA expression levels of IL7 were lower than IL8 in kidney 3 h post-injection. These results suggest that the two IL molecules play an important role in the inflammatory response of T. ovatus.
dc.format.extent10 pages
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/57044
dc.subjectTrachinotus ovatus
dc.subjectinterleukin-7 (IL7)
dc.subjectinterleukin-8 (IL8)
dc.subjectPhotobacterium damselae
dc.subjectgene expression
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture
dc.titleMolecular Characterization and Possible Immune Function of Two Members of Interleukin Family from Trachinotus ovatus
dc.typeArticle
dc.type.dcmiText

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Volume 69, 2017