Expression analysis of three immune genes IL-8, IL-6 and IL-1β in the Japanese flounder (Paralichthys olivaceus)

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dc.contributor.authorPan, Qiuli
dc.contributor.authorZhang, Feng
dc.contributor.authorLiu, Yang
dc.contributor.authorZang, Lin
dc.contributor.authorWang, Xiuli
dc.contributor.authorQiu, Xuemei
dc.date.accessioned2021-12-28T18:44:29Z
dc.date.available2021-12-28T18:44:29Z
dc.date.issued2021
dc.description.abstractInterleukin-8 (IL-8), Interleukin-6 (IL-6), and Interleukin-1β (IL-1β) are major immune-related genes that play important roles in the innate immune system of vertebrates. In this study, the expression levels of the three immune-related genes in eleven tissues of normal adult Japanese flounder (Paralichthys olivaceus) were examined using semi-quantitative RT-PCR at 13time points after challenge with Lipopolysaccharide (LPS). The results showed that the highest expression levels of the Japanese flounder IL-8 were detected at 12h in the spleen, 12h in the head kidney, and 24h in the liver after the challenge with LPS. Interestingly, the expression levels of IL-6 in the spleen and head kidney were highest at 48h. The liver had the highest expression level of IL-6 at 36h.The highest expression levels of IL-1β were detected at 3h in the spleen and 9h in the head kidney and liver. These results indicated that IL-8, IL-6, and IL-1β also played important roles in the immune response of the Japanese flounder at lower temperatures. The study provided a basis for further research on the immune mechanisms of IL-8, IL-6, IL-1β, and the production of recombinant IL-8, IL-6, or IL-1β used in Japanese flounder cultivation.
dc.format.extent12 pages
dc.identifier.doihttps://doi.org/10.46989/001c.31194
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/65089
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectParalichthys olivaceus
dc.subjectIL-8
dc.subjectIL-6
dc.subjectIL-1β
dc.subjectLPS
dc.subjecttissue expression
dc.subjectJapanese flounder
dc.subjectimmune genes
dc.titleExpression analysis of three immune genes IL-8, IL-6 and IL-1β in the Japanese flounder (Paralichthys olivaceus)
dc.typeArticle
dc.type.dcmiText
prism.volume73

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Volume 73, 2021