Transcriptome characterization through the generation and analysis of expressed sequence tags: Factors to consider for a successful EST project

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dc.contributor.authorLiu, Zhanjiang
dc.date.accessioned2012-01-19T20:18:45Z
dc.date.available2012-01-19T20:18:45Z
dc.date.issued2006
dc.description.abstractSequencing of expressed sequence tags (ESTs) has been one of the primary techniques used for transcriptome analysis in a wide spectrum of species including those utilized for aquaculture. However, many researchers are still unfamiliar with the many genome applications and byprod- ucts of EST analysis, and they often regard ESTs as only short random sequences. Additionally, lack of cDNA library normalization, project planning, and quality control have often restricted the value of EST resources created in many laboratories. This review aims to address these issues by providing information on the construction of normalized cDNA libraries for efficient generation and analysis of ESTs and by highlighting the significance of EST analysis for the genome research of species lacking a sequenced genome. A successful EST project can provide the sequence tools needed for not only gene discovery, but also for physical, linkage and compara- tive mapping, analysis of alternative splicing and gene duplication, and microarray development.
dc.format.extent13 pages
dc.identifier.citationLiu, Z. (2006). Transcriptome characterization through the generation and analysis of expressed sequence tags: Factors to consider for a successful EST project. The Israeli Journal of Aquaculture - Bamidgeh, 58(4), 328-340.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19191
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectcDNA, EST, normalization, fish, catfish, library
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleTranscriptome characterization through the generation and analysis of expressed sequence tags: Factors to consider for a successful EST project

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IJA Volume 58, Issue 4, 2006