Characterization of Vibrio alginolyticus Isolates from Diseased Cultured Gilthead Sea Bream, Sparus aurata
Date
2008
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Israeli Journal of Aquaculture - BAMIGDEH
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Abstract
Biochemical, pathogenical, antigenical, and molecular characteristics of Vibrio alginolyticus iso- lates from diseased cultured gilthead sea bream juveniles (4 and 20 g) were determined by lab- oratory challenge experiments. Intraperitonal challenge resulted in development of the disease but immersion did not. Electrophoresis of outer membrane protein (OMP) and lipopolysaccha- rides (LPS) was performed using sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE). Molecular typing of Vibrio isolates was conducted using random amplification of polymorphic DNA (RAPD). According to SDS-PAGE of surface antigens of the isolates, profiles of both OMP and LPS were heterogeneous. According to RAPD, isolated bacteria varied genet- ically. The findings reveal differences among V. alginolyticus isolates from diseased cultured gilt- head sea bream in Turkey.
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Keywords
gilthead sea bream, Vibrio alginolyticus, electrophoresis, RAPD, Fish culture--Israel--Periodicals., Fish culture--Periodicals., Aquaculture--Israel--Periodicals., Aquaculture--Periodicals.
Citation
Akayli, T., Timur, G., Aydemir, B., Kiziler, A.R., Coskun, O., Albayrak, G., & Arican, E. (2008). Characterization of Vibrio alginolyticus Isolates from Diseased Cultured Gilthead Sea Bream, Sparus aurata. The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 89-94.
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6 pages
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