Aeromonas hydrophila induces apoptosis in pufferfish, Takifugu obscurus
Date
2017
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Abstract
Aeromonas hydrophila is a Gram-negative opportunistic pathogen causing motile aeromonad septicemia, which results in great economic losses in freshwater fish farming. In this study, we investigated the effect of A. hydrophila on apoptosis of pufferfish blood cells. Total blood cell count, reactive oxygen species (ROS) production, cytoplasmic free-Ca2+ (cf-Ca2+) concentration, nitric oxide (NO) production, apoptotic cell ratio and the transcription levels of caspase-3 in the pufferfish blood cells were determined after A. hydrophila infection. Results showed that A. hydrophila infection significantly depressed the total blood cell count, and significantly increased ROS production, cf-Ca2+ concentration, NO production and apoptotic cell ratio. The transcription levels of caspase-3 increased significantly after A. hydrophila infection. Taken together, our results suggest that A. hydrophila infection alters ROS, cf-Ca2+, NO levels, and active caspase-3, which lead to cell apoptosis.
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Takifugu obscurus, Aeromonas hydrophil, apoptosis; caspase-3, Fish culture--Israel., Fish culture
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11 pages
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