Effects of dietary <em>Lactobacillus rhamnosus</em> supplementation on growth performance, antioxidant properties, and gut microbiota alterations on Chinese mitten crab, <em>Eriocheir Sinensis</em>
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2024
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76
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Numerous studies have demonstrated that Lactobacillus rhamnosus exerts growth-promoting effects in animal husbandry. However, its role in the growth performance of the Chinese mitten crab remains inadequately evaluated. In this study, we aimed to investigate the potential effects of L. rhamnosus on the growth performance, intestinal function, and microenvironment of the Chinese mitten crab. Crabs with an average weight of 0.80 ± 0.12 g were divided into four groups, with the experimental groups receiving dietary supplements of L. rhamnosus at concentrations of 0% (C group), 2% (S-1 group), 5% (S-2 group), and 8% (S-3 group), each containing 10⁹ CFU/g added to the basal diet. The results indicated that the final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) of crabs in the S-1 and S-2 groups were significantly higher than those in the control group (P < 0.05). The enzymatic activity of immune-related enzymes, including alkaline phosphatase (AKP), superoxide dismutase (SOD), and catalase (CAT), increased following dietary supplementation with L. rhamnosus. Principal coordinates analysis revealed distinct microbiota compositions between the groups. The alpha diversity indices, including ACE, Chao1, Shannon, and Sobs, were enhanced after dietary supplementation, while the Simpson index showed no significant differences. Statistical analysis demonstrated that including L. rhamnosus in the diet increased the abundance of Proteobacteria and Firmicutes while reducing the abundance of Actinobacteriota in the intestines compared to the control group. Additionally, the abundance of Paracoccus and Thiothrix in the crabs' intestines was elevated. In conclusion, the dietary inclusion of L. rhamnosus improved growth performance, enhanced intestinal microflora, and bolstered non-specific immunity and antioxidant functions in the Chinese mitten crab.
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Eriocheir Sinensis, Lactobacillus rhamnosus, Growth performance, Intestinal microbiota
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The Israeli Journal of Aquaculture - Bamidgeh
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