Effect of Different Antioxidants on Motility, Viability and Fertilizing Capacity of Cryopreserved Scaly Carp (Cyprinus carpio) Semen

Abstract

The present study investigated the effect of different antioxidants such as taurine (25 mM, 50 mM, 75 mM and 100 mM), bovine serum albumine (BSA) (1%, 2.5%, 5%, 7.5%), and vitamin C (ascorbic acid) (2.8 mM, 5.6 mM, 8.4 mM and 11.2mM), on the motility and fertilizing ability of cryopreserved scaly carp (Cyprinus carpio) semen. The results demonstrated that addition of taurine at doses of 50 mM and 75 mM, and also BSA at doses of 2.5% and 5%, improved fertilization ability of cryopreserved scaly carp semen. The highest post-thaw motility (42.4±1.5%), viability (67.8±2.6%), fertilization rate (59.3±0.5%), and also hatching rates (68.2±3.4%) were determined by supplementation of 75 mM taurine (p<0.05). These results suggest that supplementation of ionic extender with taurine is suitable for scaly carp semen cryopreservation.