Activity of the Digestive Protease, Chymotrypsin, in Larvae of the Cultured Sharpsnout Sea Bream (Diplodus puntazzo)

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dc.contributor.authorAktulun, Sevim
dc.contributor.authorSüzer, Cüneyt
dc.contributor.authorKamaci, H. Okan
dc.contributor.authorCoban, Deniz
dc.contributor.authorSaka, Sahin
dc.contributor.authorFirat, Kursat
dc.date.accessioned2012-01-19T20:23:23Z
dc.date.available2012-01-19T20:23:23Z
dc.date.issued2008
dc.description.abstractSpecific and total activity of the digestive protease, chymotrypsin, was studied in cultured sharp- snout sea bream larvae (Diplodus puntazzo) for 35 days from hatching and in a variety of pH lev- els. Activity was detected upon hatching (2.8±0.34 mm total length), before the onset of exoge- nous feeding. Specific chymotrypsin activity exponentially increased from mouth opening on day 3 until day 25, then dropped until the end of the experiment. Total chymotrypsin activity sharply increased to day 10, then continued to increase, but slightly, until the end of the experiment. As expected, pH strongly affected both specific and total chymotryptic activity in the digestive tracts of larvae. Both were significantly lower when pH was acidic (1.5, 3.0, 4.0) than when it was alka- line (8.0, 9.0, 10.0) although there were no significant differences within either the acidic or the alkaline range. Our results indicate that chymotrypsin activity is capable of digesting protein before mouth opening and may be a valuable tool for better understanding the nutritional capa- bilities of young larvae.
dc.format.extent7 pages
dc.identifier.citationAktulun, S., Suzer, C., Kamaci, H.O., Coban, D., Saka, S., & Firat, K. (2008). Activity of the Digestive Protease, Chymotrypsin, in Larvae of the Cultured Sharpsnout Sea Bream (Diplodus puntazzo). The Israeli Journal of Aquaculture - Bamidgeh, 60(2), 82-88.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19254
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectsharpsnout sea bream larvae, Diplodus puntazzo, chymotrypsin, digestive enzyme, pH
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleActivity of the Digestive Protease, Chymotrypsin, in Larvae of the Cultured Sharpsnout Sea Bream (Diplodus puntazzo)

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IJA Volume 60, Issue 2, 2008