Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm

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dc.contributor.authorBozkurt, Yusuf
dc.contributor.authorYavaş, İlker
dc.contributor.authorÖğretmen, Fatih
dc.contributor.authorSivaslıgil, Buğra
dc.contributor.authorKaraca, Fikret
dc.date.accessioned2014-06-09T20:38:44Z
dc.date.available2014-06-09T20:38:44Z
dc.date.issued2011
dc.description.abstractThis research investigated the effect of adding glycerol to different ionic extenders on motility and fertilizing ability of frozen-thawed sperm of grass carp (Ctenopharyngodon idella) under hatchery conditions. Semen was collected by abdominal stripping from adult males and diluted to a ratio of 1:3 in Kurokura I or Kurokura II extender containing 10%, 15%, or 20% glycerol. Diluted semen was packaged in 0.5-ml straws and left to equilibrate for 30 min at 4°C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196°C). Straws were thawed in a water bath at 30°C for 10 s to determine the post-thaw motility and movement duration. The spermatozoa:egg ratio in fertilization experiments was 1 x 105:1. The highest mean fertilization rate (95.63±0.52; p>0.05) was obtained with semen frozen in Kurokura I extender containing 10% glycerol. Results indicate that grass carp sperm can be successfully cryopreserved with ionic extenders containing glycerol at 10-20%.
dc.format.extent6 pages
dc.identifier.citationYusuf Bozkurt, İlker Yavaş, Fatih Öğretmen, Buğra Sivaslıgil, Fikret Karaca (2011). Effect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm. The Israeli Journal of Aquaculture - Bamidgeh, 63, 6 pp.
dc.identifier.issn0792-156X
dc.identifier.otherIIC:63.2011.635
dc.identifier.urihttp://hdl.handle.net/10524/36291
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectgrass carp
dc.subjectCtenopharyngodon idella
dc.subjectcryopreservation
dc.subjectglycerol
dc.subjectextender
dc.subjectsemen
dc.subjectfertilization
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleEffect of Glycerol on Fertility of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm

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IJA Volume 63, 2011