Multiplex Taqman Real-Time Pcr For Detecting Aeromonas Hydrophila, A. Veronii and A. Schubertii
Date
2017
Contributor
Advisor
Department
Instructor
Depositor
Speaker
Researcher
Consultant
Interviewer
Narrator
Transcriber
Annotator
Journal Title
Journal ISSN
Volume Title
Publisher
Volume
Number/Issue
Starting Page
Ending Page
Alternative Title
Abstract
In this study, pathogenic Aeromonas hydrophila, A. veronii, and A. schubertii from fish were detected by multiplex TaqMan real-time PCR assay. The assay utilized three pairs of specific primers and three corresponding TaqMan probes designed to detect the aerolysin gene in A. hydrophila, the aerolysin gene in A. veronii, and the gyrB gene in A. schubertii. The specificity of the probe and primers was evaluated. The detectable concentration for the multiplex real-time PCR was 3.33×10^1 copies/μL per reaction, respectively. In addition, the coefficient of variation was less than 1.5% for both intra-and inter-assay. The assay, when screened for 120 cultured fish samples, showed 76.7% positive for Aeromonas spp. and could accurately identify these bacterial strains. These results indicated that this assay could be used as an effective tool for rapid detection and epidemiology investigation of A. hydrophila, A. veronii, and A. schubertii.
Description
Keywords
multiplex TaqMan, real-time PCR, Aeromonas hydrophila4Aeromonas veronii, Aeromonas schubertii, Fish culture--Israel., Fish culture
Citation
Extent
9 pages
Format
Geographic Location
Time Period
Related To
Related To (URI)
Table of Contents
Rights
Rights Holder
Local Contexts
Collections
Email libraryada-l@lists.hawaii.edu if you need this content in ADA-compliant format.