Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

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dc.contributor.authorAkayli, T.
dc.contributor.authorTimur, G.
dc.contributor.authorAlbayrak, G.
dc.contributor.authorAydemir, B.
dc.date.accessioned2012-01-19T20:27:08Z
dc.date.available2012-01-19T20:27:08Z
dc.date.issued2010
dc.description.abstractThe phenotypical, serological, and molecular identification of Vibrio ordalii isolates recovered from diseased gilthead sea bream (Sparus aurata) in the Mediterranean area were investigated. Most isolates had similar phenotypic characteristics, but some showed small differentiation in biochemical analysis. Both slide agglutination and enzyme linked immunosorbent assay (ELISA) showed that the isolates were antigenically homogeneous and no different serotypes were detected. SDS-PAGE analysis showed that the structure of lipopolysaccharide (LPS) was heterogeneous, that outer membrane proteins (OMP) display homogeneous grouping protein banding patterns, and that the LPS profile of the isolates had different banding patterns. However, random amplified polymorphic DNA (RAPD) analysis revealed genetic variation (up to 90.4%) among isolates of the same species.
dc.format.extent10 pages
dc.identifier.citationAkayli, T., Timur, G., Albayrak, G., & Aydemir, B. (2010). Identification and Genotyping of Vibrio ordalii: A Comparison of Different Methods. The Israeli Journal of Aquaculture - Bamidgeh, 62(1), 9-18.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19305
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectgilthead sea bream, Vibrio ordalii, serology, SDS-PAGE, RAPD
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleIdentification and Genotyping of Vibrio ordalii: A Comparison of Different Methods

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IJA Volume 62, Issue 1, 2010