Effects of Dietary Astaxanthin on Growth Performance, Hepatic Antioxidative Activity, hsp70, and HIF-1α Gene Expression of Juvenile Golden Pompano (Trachinotus ovatus)
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2017
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Abstract
The present study was conducted to investigate the effects of different astaxanthin (AST) levels on growth performance, hepatic antioxidative activity, heat shock protein 70 (hsp70), and hypoxia-inducible factor-1 alpha (HIF-1α) gene expression of juvenile golden pompano. Fish (mean initial body weight 5.8±0.05g) were fed six isonitrogenous and isoenergetic diets (AST-0, AST-0.005, AST-0.01, AST-0.05, AST-0.1, AST-0.2) containing various supplemented levels of astaxanthin (0%, 0.005%, 0.01%, 0.05%, 0.1%, 0.2%, respectively) in triplicate for 8 weeks. Growth performance (final body weight, FBW; weight gain, WG), and survival of fish fed diets containing astaxanthin above 0.01% were significantly higher (P<0.05) than of fish fed AST-0 and AST-0.005 diets; feed conversion ratio (FCR) showed the opposite trend (P<0.05). Fish fed 0.01% astaxanthin diet showed the highest value of survival. Hepatic antioxidant status (total antioxidant status, TAS; superoxide dismutase, SOD; carbonyl protein content) of shrimp improved significantly when dietary astaxanthin was over 0.01%. Relative expression profiles of hepatic hsp 70 mRNA and HIF-1α mRNA increased with increased dietary astaxanthin levels. The relative expression profiles of hepatic hsp 70 mRNA and HIF-1α mRNA of shrimp fed diets containing astaxanthin above 0.01% were obviously higher than those of shrimp fed AST-0 and AST-0.005 diets. Linear regression analysis on WG and HIF-1a mRNA indicated that the optimum dietary AST levels for optimal growth and immunity of juvenile pompano were 0.011% and 0.013%, respectively.
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Trachinotus ovatus, astaxanthin, growth, surviva, immunity, Fish culture--Israel., Fish culture
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13 pages
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