Development of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active <em>Streptococcus dysgalactiae</em> emerging in channel catfish (<em>Ictalurus punctatus</em>)

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dc.contributor.authorWang, Jiao
dc.contributor.authorFeng, Yang
dc.contributor.authorFan, Wei
dc.contributor.authorPeng, Kun
dc.contributor.authorZhou, Keyu
dc.contributor.authorChen, Defang
dc.contributor.authorHuang, Xiaoli
dc.contributor.authorOuyang, Ping
dc.contributor.authorGuo, Hongrui
dc.contributor.authorLai, Weimin
dc.contributor.authorGeng, Yi
dc.date.accessioned2024-10-31T20:49:45Z
dc.date.available2024-10-31T20:49:45Z
dc.date.issued2024
dc.description.abstract<em>Streptococcus dysgalactiae</em> has become an important pathogen affecting mammals and fish (especially farmed fish). The development of a rapid, accurate, and sensitive diagnostic method will aid in the prevention of <em>S. dysgalactiae</em> infection. In this study, a method combining recombinase-aided amplification and a lateral flow dipstick (RAA-LFD) was developed to detect active <em>S. dysgalactiae</em> infection. The RAA-LFD detection was based on the <em>ISP</em> gene of <em>S. dysgalactiae</em> as the target, which could be accomplished with the lowest detection limit of 1.002×10^2^ pg/μL reaction under optimal reaction conditions of 39 °C for 15 min. RAA-LFD and conventional PCR were used to detect 50 experimental samples invaded by <em>S. dysgalactiae</em> disease materials. The results showed that the positive concordance rate of the two methods was 97.6%, the negative concordance rate was 90.0%, and the total concordance rate was 98.0%. Compared with conventional PCR, the Area Under the ROC Curve (AUC) of RAA-LFD was 0.950, and the sensitivity and specificity were 100% and 90%, respectively. The RAA-LFD of <em>S. dysgalactiae</em> infection is convenient for rapid clinical detection.
dc.identifier.doihttps://doi.org/10.46989/001c.125006
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/78025
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectStreptococcus dysgalactiae
dc.subjectrapid detection
dc.subjectrecombinase-aided amplification
dc.subjectlateral-flow dipstick assay
dc.subjectinfection.
dc.titleDevelopment of a Lateral Flow Strip-Based Recombinase-Aided Amplification for Active <em>Streptococcus dysgalactiae</em> emerging in channel catfish (<em>Ictalurus punctatus</em>)
dc.typeArticle
dc.type.dcmiText
prism.volume76

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Volume 76, Issue 4, 2024