Production Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction

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dc.contributor.authorBen-Dom, Naomi
dc.contributor.authorCherfas. Nina B.
dc.date.accessioned2012-01-19T20:07:00Z
dc.date.available2012-01-19T20:07:00Z
dc.date.issued2001
dc.description.abstractThe production of two heterozygous and one homozygous clones of the Israeli Dor-70 line of com- mon carp (Cyprinus carpio L.) was tested using DNA fingerprinting (DFP) and mixed leukocyte reaction (MLR) analysis. The clones were obtained in large-scale production from mitotic gyno- genetic females and males (sex-inversed). MLR analysis was used to examine major histocom- patibility complex (MHC) antigens in individuals. DFP provided evidence concerning identity in the whole genome. Both methods showed that individuals within a progeny were identical, confirming genetic uniformity within each clone and showing that no paternal DNA was transmitted during mitotic and meiotic gynogenesis. The results indicated that uniformity of DFP in progeny of the homozygous clone is obtained only when the mother is fully homozygous, and in the heterozygous clone only if both parents are fully homozygous. The results of both methods provide evidence, though indirect, that the mitotic gynogenetic parents of these fish (mother of the homozygous clone, mother and father of the heterozygous clones) were fully homozygous.
dc.format.extent12 pages
dc.identifier.citationBen-Dom, N., & Cherfas, N.B. (2001). Production Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction. The Israeli Journal of Aquaculture - Bamidgeh, 53(3 - 4), 89-100.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19034
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectcommon carp, Cyprinus carpio, DNA fingerprinting, heterozygosity, homozygosity, mixed leukocyte reaction
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleProduction Of Heterozygous And Homozygous Clones Of Common Carp (Cyprinus Carpio L.): Evidence From DNA Fingerprinting And Mixed Leukocyte Reaction

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IJA Volume 53, Issue 3-4, 2001