Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens

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dc.contributor.authorNayak, S.K.
dc.contributor.authorMukherjee, S.C.
dc.date.accessioned2014-06-09T20:38:50Z
dc.date.available2014-06-09T20:38:50Z
dc.date.issued2011
dc.description.abstractThe present investigation was carried out to study the active component of Bacillus subtilis, a gastrointestinal bacterium of mrigal (Cirrhinus mrigala) that shows inhibitory activity against a wide range of fish pathogens. The bacterium was found to inhibit all the fish pathogens and an extra cellular protein fraction (ECP) of 5-10 kD was found to be the active component responsible for inhibitory activity. The component was heat stable (60°C for 1 h) and a change in pH had no effect on the antibacterial activity. The protein fraction was sensitive to trypsin, chymotrypsin, lysozyme, and proteinase K at 2.5 mg/ml and resistant at 0.1 and 0.5 mg/ml. The present investigation confirmed that the active component of the B. subtilis responsible for antibacterial activities against common fish pathogens is a low molecular weight protein fraction (5-10 kD). A protein or peptide with such a low molecular weight has the potential to serve as an alternative health management strategy for combating disease in aquaculture.
dc.format.extent5 pages
dc.identifier.citationS.K. Nayak and S.C. Mukherjee (2011). Partial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens. The Israeli Journal of Aquaculture - Bamidgeh, 63, 5 pp.
dc.identifier.issn0792-156X
dc.identifier.otherIIC:63.2011.648
dc.identifier.urihttp://hdl.handle.net/10524/36292
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectantimicrobial activity
dc.subjectBacillus subtilis
dc.subjectextra cellular protein (ECP) fraction
dc.subjectfish pathogens
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titlePartial Purification and Characterization of the Inhibitory Substance of Bacillus subtilis against Common Bacterial Fish Pathogens

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