The Molecular Characterization of the Cyprinid herpesvirus 3 (CyHV-3) ORF24 Protein and its effect on the expression of immune genes (in vitro)

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dc.contributor.authorWang, Jing
dc.contributor.authorJi, Yan
dc.contributor.authorZhou, Xuan
dc.contributor.authorYu, Denghang
dc.contributor.authorTan, Kianann
dc.contributor.authorZhang, Chi
dc.date.accessioned2024-03-04T21:09:56Z
dc.date.available2024-03-04T21:09:56Z
dc.date.issued2024
dc.description.abstractCyprinid herpesvirus 3 (CyHV-3), known as koi herpesvirus (KHV), is highly contagious and lethal. In this study, we aimed to characterize the ORF24-encoding protein of CyHV-3, investigate its sub-cellular localization, and determine its impact on the expression of immune factors through in vitro experiments. The results showed that the CyHV-3 ORF24 protein comprises 579 amino acids. Interestingly, multiple comparisons with homologous proteins from three carp herpesvirus origins showed no significant similarity. The fluorescence localization experiment showed that the green fluorescence signal, representing the protein pEGFP-ORF24, was primarily diffused in the cytoplasm. Notably, the overexpression of ORF24 effectively suppressed the expression of immune factors in both CCO (Channel catfish ovary) and FHM (Fathead minnow muscle cell line) cells. Bioinformatic analysis indicated that the CyHV-3 ORF24 gene exhibited significant differences from the corresponding genes in the other two carp herpesviruses. This suggests its unique functional role in the evolutionary context. Moreover, our findings demonstrated that overexpression of CyHV-3 ORF24 can effectively inhibit the expression of immune factors, underscoring its crucial role as a viral immune escape factor. These results provide further insights into the immune function of the CyHV-3 ORF24 protein and offer a theoretical foundation for developing new vaccines against CyHV-3 virus infections.
dc.identifier.doihttps://doi.org/10.46989/001c.94381
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/75376
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectCyHV-3
dc.subjectBioinformatics analysis
dc.subjectProtein properties
dc.subjectImmune factors
dc.subjectVaccines
dc.titleThe Molecular Characterization of the Cyprinid herpesvirus 3 (CyHV-3) ORF24 Protein and its effect on the expression of immune genes (in vitro)
dc.typeArticle
dc.type.dcmiText
dspace.entity.type
prism.volume76

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Volume 76, Issue 1, 2024