An Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas

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dc.contributor.authorZhang, Guofan
dc.contributor.authorWang, Xiaotong
dc.contributor.authorSong, Xiaorui
dc.contributor.authorLi, Li
dc.date.accessioned2012-10-15T23:33:08Z
dc.date.available2012-10-15T23:33:08Z
dc.date.issued2012
dc.description.abstractWe earlier developed a method to extract DNA from in vivo-sampled mantle tissue of the bivalve Pacific oyster (Crassostrea gigas) for molecular marker-assisted selection (MAS) in breeding. However, mortality was too high when using this method. In the current study, a more efficient and safer method of extracting DNA from the oyster shell was developed, based on the improved phenol-chloroform DNA extraction method. Both nuclear DNA and mitochondrial DNA were successfully extracted from the oyster shell, and mortality was 0%. These results illustrate the safety of extracting DNA from the bivalve shell. In addition, because DNA is a cellular component, the results provide molecular evidence that cells are likely involved in shell formation.
dc.format.extent4 pages
dc.identifier.issn0792-156X
dc.identifier.otherIJA_64.2012.800
dc.identifier.urihttp://hdl.handle.net/10524/31832
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectmollusc
dc.subjectbivalve
dc.subjectPacific oyster
dc.subjectshell DNA
dc.subjectmarker-assisted selection (MAS)
dc.subject.lcshFish culture--Israel.
dc.subject.lcshFish culture.
dc.titleAn Improved Method of DNA Extraction from the Shell of the Pacific Oyster, Crassostrea gigas

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Volume 64, 2012