Genome-wide identification and expression analysis of Bcl-2 gene family under low-temperature stress in tilapia (Oreochromis niloticus)

Simple item page
dc.contributor.authorYang, Changgeng
dc.contributor.authorWang, Meizi
dc.contributor.authorWen, Hua
dc.contributor.authorJiang, Ming
dc.contributor.authorTian, Juan
dc.contributor.authorLu, Xing
dc.date.accessioned2021-03-01T19:13:22Z
dc.date.available2021-03-01T19:13:22Z
dc.date.issued2020
dc.description.abstractLow temperature stress can lead to variety of changes, including apoptosis in tilapia (Oreochromis niloticus). The B cell lymphoma-2 (Bcl-2) gene family plays an important role in the process of apoptosis. The present study conducted genome-wide characterization of the Bcl-2 family genes in tilapia and their mRNA expression profiles were analysed in different tissues of tilapia under the low temperature stress (10°C). Twenty-four Bcl-2 family genes were identified, containing 2~8 exons. These genes were classified into two subfamilies (Bcl-2 homologs and BH3-only) based on their conserved domains. Besides, these BCL-2 proteins in tilapia possess at least one of the four conserved BH domains. The phylogenetic analysis showed that the Bcl-2 family genes did not aggregate by species, demonstrating sequence conservation of different types of Bcl-2 family members. Real-time quantitative PCR (RT-qPCR) analysis showed that Bcl-2 family genes were broadly expressed in different tissues of tilapia. When reared at 10 °C, the transcriptional expression levels of most of anti-apoptotic Bcl-2 homologs subgroup members and other BH3-only subgroup members in most tissues of tilapia were higher than those at 30°C. However, most of other Bcl-2 family members revealed a lower expression. The results suggested that hypothermia had significantly induced apoptotic in tilapia.
dc.format.extent14 pages
dc.identifier.doihttps://doi.org/10.46989/001c.21307
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/63787
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectapoptosis
dc.subjectb cell
dc.subjectgenome
dc.subjectexpression profile
dc.subjectstress
dc.subjectlow temperature stress
dc.subjectphylogeny
dc.subjectbcl-2
dc.subjecttilapia
dc.subjectoreochromis niloticus
dc.titleGenome-wide identification and expression analysis of Bcl-2 gene family under low-temperature stress in tilapia (Oreochromis niloticus)
dc.typeArticle
dc.type.dcmiText
prism.volume72

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
21307.pdf
Size:
1.52 MB
Format:
Adobe Portable Document Format
Download

Collections

Volume 72, 2020