Intestinal fatty acid binding protein gene (I-FABP) in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae: ontogenetic expression and response to water temperature and nutrition manipulation

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dc.contributor.author Lin, M.
dc.contributor.author Tang, X.
dc.contributor.author Qin, J.G.
dc.contributor.author Ma, Z.
dc.contributor.author Wei, W
dc.date.accessioned 2017-12-28T02:34:43Z
dc.date.available 2017-12-28T02:34:43Z
dc.date.issued 2017
dc.description.abstract The gene for fatty acid binding proteins (I-FABP) in golden pompano Trachinotus ovatus larvae was cloned and analyzed from hatch to 18 days-post hatch (DPH). The I-FABP gene (GenBank accession: MF034871) of golden pompano is composed of 815 bp with an open reading frame of 399 bp, encoded in one amino acid with a molecular weight of 15.24 kDa. The predicted amino acid sequence of I-FABP genes from golden pompano showed high similarity and identity with Japanese sea bass Lateolabrax japonicus (97% and 87.9%, AOW69620.1). The highest tissue expression of I-FABP genes was found in the intestine, followed by the eye on 18 DPH. During the ontogenetic development, the expression of I-FABP genes remained at a low level during the first five days, and reached the highest level on 12 and 18 DPH. The expression of I-FABP genes was not significantly affected by environmental temperature on 12 DPH, but was significantly affected by the temperature on 18 DPH. Nutrition enhancement with algae containing high fatty acids significantly affected the expression of I-FABP genes. The highest expression was observed in the non-enriched treatment, but the lowest expression was in the Nannochloropsis feeding treatment. Results of the present study indicate that the expression of the I-FABP gene varies with environmental temperature and nutritional conditions during the ontogenetic development of golden pompano larvae. The expression of I-FABP genes may be potentially used as an indicator for assessing nutrient supply and functional development of the digestive system in fish larvae.
dc.format.extent 13 pages
dc.identifier.issn 0792-156X
dc.identifier.uri http://hdl.handle.net/10524/57056
dc.subject I-FABP
dc.subject golden pompano
dc.subject ontogenetic expression
dc.subject nutrition manipulation
dc.subject.lcsh Fish culture--Israel.
dc.subject.lcsh Fish culture
dc.title Intestinal fatty acid binding protein gene (I-FABP) in golden pompano Trachinotus ovatus (Linnaeus 1758) larvae: ontogenetic expression and response to water temperature and nutrition manipulation
dc.type Article
dc.type.dcmi Text
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