Effects Of Dichlorvos And Characterization Of Microsomal Nadph-Cytochrome P450 And NADH-ferricyanide Reductase In Rainbow Trout (Oncorhynchus Mykiss) Liver

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dc.contributor.authorÖzen, Tevfik
dc.contributor.authorKorkmaz, Halil
dc.date.accessioned2012-01-19T20:12:04Z
dc.date.available2012-01-19T20:12:04Z
dc.date.issued2004
dc.description.abstractThe kinetics of oxidation of rainbow trout (Oncorhynchus mykiss) NADPH-cytochrome P450 reductase (NCPR) by cytochrome-c and NADH-ferricyanide reductase (NFR) by K3Fe(CN)6 were studied. NCPR and NFR activities from liver microsomes of rainbow trout (Oncorhynchus mykiss) had a specific activity of 0.0246 and 0.0350 nmoles/min/mg protein, respectively. The maximal rate of NCPR reaction was found at cytochrome-c concentrations above 2.1 mM and in the presence of cyt c. Vmax and Km were 0.0083 nmoles/min/mg and 1.14 mM. The maximal rate of NFR reaction was at K3Fe(CN)6 concentrations higher than 2.0 mM and in the presence of K3Fe(CN)6. Vmax and Km values were 1.04 nmoles/min/mg and 0.352 mM, respectively. The inhi- bition by dichlorvos (DDVP) on NCPR and NFR activity was not exhibited over the range 1.0-5.0 mM DDVP.
dc.format.extent10 pages
dc.identifier.citationÖzen, T., & Korkmaz, H. (2004). Effects Of Dichlorvos And Characterization Of Microsomal Nadph-Cytochrome P450 And NADH-ferricyanide Reductase In Rainbow Trout (Oncorhynchus Mykiss) Liver. The Israeli Journal of Aquaculture - Bamidgeh, 56(1), 35-44.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19098
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectDDVP, microsomes, NADPH-cytochrome P450 reductase, NADH-ferricyanide reductase, Oncorhynchus mykiss
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleEffects Of Dichlorvos And Characterization Of Microsomal Nadph-Cytochrome P450 And NADH-ferricyanide Reductase In Rainbow Trout (Oncorhynchus Mykiss) Liver

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IJA Volume 56, Issue 1, 2004