Studies on the resistance of three pathogens isolated from wild <em>Charybdis japonica</em> and their pathogenicity in <em>Portunus trituberculatus</em>

Simple item page
dc.contributor.authorQin, Yawei
dc.contributor.authorTan, Yanmiao
dc.contributor.authorLiu, Yufeng
dc.contributor.authorHe, Zhongwei
dc.contributor.authorHan, Tian
dc.contributor.authorYang, Tianlei
dc.contributor.authorBai, Zishuo
dc.contributor.authorWang, Jianmeng
dc.contributor.authorGong, Chunguang
dc.date.accessioned2024-11-25T20:58:57Z
dc.date.available2024-11-25T20:58:57Z
dc.date.issued2024
dc.description.abstractThe coagulation property of hemolymph is one of the characteristic indicators of the immune function of crustaceans. This article focuses on the abnormal phenomenon of wild <em>Charybdis japonica,</em> where the hemolymph cannot coagulate in the Qinhuangdao Sea area. The pathogens were isolated and purified, and three dominant strains, N1, N2, and Q1, were obtained. These strains were identified as <em>Vibrio natriegens</em>, <em>V. fortis</em>, and <em>Metschnikowia bicuspidata</em> through morphological observation, physiological and biochemical experiments, and 16S rDNA or ITS sequence analysis. Subsequently, drug sensitivity and probiotic antagonism analyses were performed. Considering the potential for cross-species transmission of crustacean pathogens, the isolated pathogens may bring potential hazards to <em>Portunus trituberculus</em>, an important aquaculture economic species in China. Therefore, artificial infection and histopathological analysis were conducted on <em>P. trituberculatus</em>. The results of drug sensitivity and antagonism assays revealed that stain <em>V. natriegens</em> exhibited susceptibilities towards antibiotics such as streptomycin and norfloxacin, but 10 probiotics had no antagonistic effect on it; strain <em>V. fortis</em> exhibited susceptibilities to antibiotics such as tetracycline and chloramphenicol, moreover, strain <em>V. fortis</em> showed inhibitory effects on <em>Bacillus velezensis</em> and <em>B. amyloliquefaciens</em>. Strain <em>M. bicuspidata</em> exhibited drug susceptibility towards polymyxin B and showed inhibitory effects on <em>B. toyonensis</em>, <em>B. velezensis</em>, and <em>B. amyloliquefaciens</em>. The results of artificial infection tests using <em>V. natriegens</em>, <em>V. fortis</em>, and <em>M. bicuspidata</em> in <em>P. trituberculatus</em> showed cumulative mortality rates of 100%, 75%, and 35%, respectively, after 120 hours of infection. The hemolymph of the deceased <em>P. trituberculatus</em> following injection with strain <em>V. natriegens</em> or <em>V. fortis</em> exhibited a light blue and did not coagulate normally. Conversely, the hemolymph of the deceased crabs following injection with strain <em>M. bicuspidata</em> appeared milky white and normal coagulation. Histopathology examination demonstrated that strains <em>V. natriegens</em>, <em>V. fortis</em>, and <em>M. bicuspidata</em> can all induce lesions in several tissues. This study is the first to isolate <em>V. natriegens</em> and <em>V. fortis</em> from crustaceans, and the infection tests on the <em>P. trituberculatus</em> can replicate the symptoms, such as hemolymph noncoagulation. Based on these findings, it is speculated that the pathogens capable of casing hemolymph noncoagulation syndrome in <em>P. trituberculatus</em> may originate from wild <em>C. japonica</em>. The drug resistance and probiotic antagonism test results can provide a theoretical basis for precise medication and offer insights into the development and application of probiotic preparations targeting the three pathogens.
dc.identifier.doihttps://doi.org/10.46989/001c.125769
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/79403
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectHemolymph noncoagulation disease
dc.subjectPathogenicity
dc.subjectResistance
dc.subjectCharybdis japonica
dc.subjectPortunus trituberculatus
dc.subjectVibrio natriegens
dc.subjectMetschnikowia bicuspidata
dc.subjectVibrio fortis
dc.titleStudies on the resistance of three pathogens isolated from wild <em>Charybdis japonica</em> and their pathogenicity in <em>Portunus trituberculatus</em>
dc.typeArticle
dc.type.dcmiText
prism.volume76

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
125769.pdf
Size:
5.36 MB
Format:
Adobe Portable Document Format
Download

Collections

Volume 76, Issue 4, 2024