Antigenic Characterization of Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus L.)

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dc.contributor.authorMali, P.
dc.contributor.authorMaji, S.
dc.contributor.authorJoardar, S.N.
dc.date.accessioned2012-01-19T20:20:28Z
dc.date.available2012-01-19T20:20:28Z
dc.date.issued2007
dc.description.abstractAeromonas sobria, along with A. hydrophila, has frequently been reported as a causative agent of motile aeromonas septicemia (MAS) in fish and other aquatic organisms. Currently, there are no precise tools to diagnose or vaccinate against this disease. The aim of the present study was to fractionate and characterize the outer membrane protein (OMP) antigen of A. sobria by sero- logical and cellular techniques so as to identify immunoreactive molecules that might be useful in preparing immunodiagnostic and/or immunoprophylactic tools against A. sobria infection in goldfish. Eight fractions were isolated from the crude OMP antigen using sephacryl S-200 and DEAE-cellulose chromatography. The highest seroreactivity was observed in the gel-permeated protein G1 which had an optical density (OD) of 0.72 nm, higher even than that of the crude OMP antigen, 0.63 nm. The serodiagnostic potential of G1 was assessed by using dip-stick ELISA. The in vitro goldfish lympho-proliferation ability of the fractionated antigen, G1A3, was higher than of all the other fractionated antigens and the crude OMP. Therefore, fractionated antigen G1 (molecular wt 42-67 kDa) and G1A3 (molecular wt 45-47 kDa) should be further studied in immunodiagnostic and/or immunoprophylactic preparations for A. sobria infection.
dc.format.extent8 pages
dc.identifier.citationMali, P., Maji, S., & Joardar, S.N. (2007). Antigenic Characterization of Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus L.). The Israeli Journal of Aquaculture - Bamidgeh, 59(2), 91-98.
dc.identifier.issn0792-156X
dc.identifier.urihttp://hdl.handle.net/10524/19215
dc.publisherIsraeli Journal of Aquaculture - BAMIGDEH
dc.relation.ispartofseriesThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectAeromonas sobria, ELISA, fractionation, outer membrane protein (OMP)
dc.subject.lcshFish culture--Israel--Periodicals.
dc.subject.lcshFish culture--Periodicals.
dc.subject.lcshAquaculture--Israel--Periodicals.
dc.subject.lcshAquaculture--Periodicals.
dc.titleAntigenic Characterization of Outer Membrane Protein of Aeromonas sobria Isolated from Goldfish (Carassius auratus L.)

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IJA Volume 59, Issue 2, 2007