Effect of Extender Supplemented with Different Sugar Types on Post-thaw Motility, Viability and Fertilizing Ability of Cryopreserved Common Carp (Cyprinus carpio) Spermatozoa
Date
2016
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Abstract
The influence of various sugar types supplemented to the extender on post-thaw motility, viability, and fertilizing capacity of cryopreserved common carp (Cyprinus carpio) semen were investigated. The results indicated that types of sugar significantly influenced motility, motility duration, and viability rates (P<0.05). Glucose, maltose, sucrose, and trehalose provided higher motility compared to the sugar-free control in post-thaw samples. Trehalose provided highest progressive motility duration, and higher viable sperm rates were obtained with all sugar types except xylose. Xylose exhibited the lowest post-thaw progressive motility duration (35.2±1.4s). The mean highest fertilization (78.2±1.4%) and eyed egg rates (94.3±1.5%) were determined using trehalose in the extender, and differences between the treatments in the fertilization and eyeing rates were significant (P<0.05). Finally, the present study showed that sugars, especially maltose and trehalose (disaccharide), improved post-thaw spermatozoa motility and fertility in common carp semen.
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cryopreservation, seme, extender, sugar, Cyprinus carpio, Fish culture--Israel., Fish culture.
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8 pages
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The Israeli Journal of Aquaculture - Bamidgeh
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