Effects of molting on the growth of primary cultured muscle cells from and Short Communication
dc.contributor.author | Wang, Wei-Na | |
dc.contributor.author | Wang, An-Li | |
dc.contributor.author | Nu, Dong-Hong | |
dc.contributor.author | Liu, Yi-Chen | |
dc.contributor.author | Sun, Ru-Yong | |
dc.date.accessioned | 2012-01-19T20:16:05Z | |
dc.date.available | 2012-01-19T20:16:05Z | |
dc.date.issued | 2005 | |
dc.description.abstract | This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 μg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant (p<0.05). | |
dc.format.extent | 7 pages | |
dc.identifier.citation | Wang, W., Wang, A., Nu, D., Liu, Y., & Sun, R. (2005). Effects of molting on the growth of primary cultured muscle cells from and Short Communication. The Israeli Journal of Aquaculture - Bamidgeh, 57(4), 264-270. | |
dc.identifier.issn | 0792-156X | |
dc.identifier.uri | http://hdl.handle.net/10524/19154 | |
dc.publisher | Israeli Journal of Aquaculture - BAMIGDEH | |
dc.relation.ispartofseries | The Israeli Journal of Aquaculture - Bamidgeh | |
dc.subject | cell culture, Macrobrachium nipponense, molt, Penaeus vannamei, RNA:DNA ratio | |
dc.subject.lcsh | Fish culture--Israel--Periodicals. | |
dc.subject.lcsh | Fish culture--Periodicals. | |
dc.subject.lcsh | Aquaculture--Israel--Periodicals. | |
dc.subject.lcsh | Aquaculture--Periodicals. | |
dc.title | Effects of molting on the growth of primary cultured muscle cells from and Short Communication |
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