Molecular Detection and Subtype Distribution of <em>Blastocystis sp.</em> in Cold-water Fish and cultured water

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dc.contributor.authorWang, Yuwei
dc.contributor.authorZhang, Chao
dc.contributor.authorNan, Huizhu
dc.contributor.authorYin, Shi
dc.contributor.authorCao, Mengjuan
dc.contributor.authorAn, Ruiyong
dc.contributor.authorMa, Lei
dc.date.accessioned2024-01-31T21:19:20Z
dc.date.available2024-01-31T21:19:20Z
dc.date.issued2024
dc.description.abstract<em>Blastocystis</em> sp. is a eukaryotic protozoan parasite reported in patients with diarrhea and irritable bowel syndrome. So far, it has been determined the parasite occurs in the intestinal tract of humans and animals worldwide. However, few studies have investigated the distribution of <em>Blastocystis</em> sp. in cold-water fish and cultured water. After DNA extraction of tissue and water samples, a polymerase chain reaction (PCR) assay was established to detect <em>Blastocystis sp.</em> The results showed the detection rate of the parasite was 3.70% in the tissue samples and 6.29% in water samples. Only the ST1 subtype was in fish, but five subtypes, ST1, ST2, ST10, ST21, and ST24, were detected in water samples. There were no significant differences in the water distribution rate of <em>Blastocystis</em> sp. The presence of <em>Blastocystis</em> sp. was detected in the intestinal digesta of cold-water fish for the first time in this study, which hinted that <em>Blastocystis</em> sp. may infect cold-water fish. It is speculated that <em>Blastocystis</em> sp. entered the intestinal tract of fish with feeding and water flow. The results will provide data support for ecological prevention and control of cold-water fish diseases and lay a foundation for the formulation of sustainable aquaculture development strategies.
dc.identifier.doihttps://doi.org/10.46989/001c.92504
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/74454
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectBlastocystis
dc.subjectfish
dc.subjectPCR
dc.subjectsubtype
dc.titleMolecular Detection and Subtype Distribution of <em>Blastocystis sp.</em> in Cold-water Fish and cultured water
dc.typeArticle
dc.type.dcmiText
dspace.entity.type
prism.volume76

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Volume 76, Issue 1, 2024