Isolation and identification of <em>Acinetobacter lwoffii</em> from the Chinese Giant Salamander (<em>Andrias davidianus</em>)

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dc.contributor.authorMao, Pan
dc.contributor.authorXie, Yixing
dc.contributor.authorWang, Cheng
dc.contributor.authorDeng, Zhiyong
dc.contributor.authorYuan, Huayan
dc.contributor.authorTian, Mingzhu
dc.contributor.authorWei, Ying
dc.contributor.authorZhou, Yong
dc.date.accessioned2024-09-13T18:18:13Z
dc.date.available2024-09-13T18:18:13Z
dc.date.issued2024
dc.description.abstractThis study aimed to identify the primary pathogenic bacteria responsible for the mortality of the Chinese giant salamander (<em>Andriasd davidianus</em>). A pathogenic bacterium was isolated from a diseased Chinese giant salamander exhibiting typical symptoms under aseptic conditions and then identified by morphological examinations, biochemical analysis, and the sequence analysis of bacterial 16S rRNA. Artificial infection testing was then conducted to determine the pathogenicity of the isolated bacteria strain. Drug susceptibility tests were conducted using the agar diffusion method. The isolated pathogenic bacteria, named DN-2, was successfully identified as <em>Acinetobacter lwoffii</em>. The artificial infection showed that the typical symptoms of the disease could be replicated as the ones originally occurred, and this bacterium exhibited high pathogenicity to the Chinese giant salamander. In the Chinese giant salamander, the median lethal dosage (LD50) of <em>A. lwoffii</em> DN-2 for A. davidianus was determined to be 4.63×104 CFU/g. Drug sensitivity testing showed that these bacteria were highly sensitive to erythromycin, gentamicin, neomycin, streptomycin, midecamycin, ciprofloxacin, piperacillin, florfenicol, doxycycline, carbenicillin, and sulfanilamide. In summary, <em>A. lwoffii</em> was identified as the primary pathogen responsible for the demise of <em>A. davidianus</em>. Our study first presents how these bacteria harm Chinese giant salamanders.
dc.identifier.doihttps://doi.org/10.46989/001c.123008
dc.identifier.issn0792-156X
dc.identifier.urihttps://hdl.handle.net/10524/77528
dc.relation.ispartofThe Israeli Journal of Aquaculture - Bamidgeh
dc.subjectAndrias davidianus
dc.subjectAcinetobacter lwoffii
dc.subjectIsolation
dc.subjectidentification
dc.titleIsolation and identification of <em>Acinetobacter lwoffii</em> from the Chinese Giant Salamander (<em>Andrias davidianus</em>)
dc.typeArticle
dc.type.dcmiText
prism.volume76

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Volume 76, Issue 3, 2024