Volume 77, Issue 2, 2025

Permanent URI for this collectionhttps://hdl.handle.net/10524/83019

Browse

Now showing 1 - 20 of 32

Study on morphological characteristics and hypoxia tolerance of Culter alburnus (♀) × Magalobrama terminalis (♂) and Culter alburnus
Cheng, Shun; Zhang, Meifang; Jiang, Wenping; Chi, Meili; Zheng, Jianbo; Liu, Shili; Hang, Xiaoying; Peng, Miao; Li, Fei
C. alburnus (♀) × M. terminalis (♂) (CM) is a new national cultivar formed by interspecific hybridization between C. alburnus and M. terminalis. To explore the morphological characteristics and hypoxia tolerance of CM and C. alburnus, quantitative and countable traits were measured for CM and C. alburnus; then we conducted hypoxia stress research. The results: 1. The main characteristics of CM that differed from C. alburnus in appearance were an increase in body height and thickness, a small blunt pointed head, bulging back of the head, and large scales on the sides of the body. Among the countable traits, the numbers of lateral scales, upper lateral scales, and first-gill rakes of CM were significantly lower than those of C. alburnus, whereas the number of lower lateral scales was significantly greater than that of C. alburnus. 2. At the water temperature of 25 ℃, the DO value of C. alburnus was higher than that of CM. The Superoxide dismutase (SOD) or glutathione (GSH) index of CM increased more when it increased and decreased less when it decreased. The SOD or GSH index of CM was higher than that of the CM under the same hypoxic stress time. Therefore, it was speculated that CM might have stronger hypoxia tolerance than C. alburnus. In summary, CM was generally similar to C. alburnus in terms of morphological characteristics; however, there were differences in body height, body thickness, tail handle, and scales. It has been speculated that CM have a stronger tolerance to hypoxia.
Gut Microbiota deliverables: reflecting the Efficacy of Dietary Probiotic Supplementation for Growth and Feed Utilization in Litopenaeus vannamei Towards Sustainable Aquaculture
(2025) Vu, Quyen D.H.; Pham, Linh P.; Vo, Trung D.; Nguyen, Le T.B.; Nguyen, Huy D.; Tran, Sang Q.; Truong, Oanh T.; Dang, Binh T.; Le, Minh-Hoang
The growing demand for sustainable aquaculture requires improvements in shrimp production efficiency and health management. This study investigated the effects of dietary Bacillus subtilis and Lactobacillus acidophilus supplementation on the gut microbiota dynamics, growth performance, survival, and feed utilization of the Pacific white shrimp (Litopenaeus vannamei) post-larvae. Shrimp were reared over 84 days under four experimental groups: control (a probiotic-free diet), supplemented with 10⁷ CFU/g Lactobacillus (L), 10⁷ CFU/g Bacillus (B), and a mixed probiotic treatment (M) with 0.5x10⁷ CFU/g per each. Growth metrics, survival rate and, feed utilization were assessed, and entire intestinal samples were analyzed using high throughput DNA sequencing of V1-V9 of 16S rRNA hypervariable regions. Bacillus supplementation significantly improved final body weight and specific growth rate (SGR) compared to the other groups (p < 0.05), leading to notably higher biomass by Day 74. However, by the end of the 84-day experiment, these significant differences in body weight and length were no longer observed among the treatment groups (p > 0.05). The mixed probiotic group demonstrated significantly better FCR than the Bacillus group (p < 0.05), indicating improved feed efficiency. Gut microbiota analysis showed dominance of Pseudomonadota at the phylum level and Vibrio at the genus level (18.06%–48.28%). Neither alpha nor beta diversity was significantly affected by probiotic treatments (p > 0.05), though temporal and treatment-specific shifts in bacterial composition were detected. These results suggest that Bacillus and mixed probiotic supplementation may enhance shrimp growth and feed efficiency, supporting their potential application in sustainable shrimp farming.
Effects of water temperature and feed iron level on nitrite resistance of spotted seabass （Lateolabrax japonicus）
(2025) Sun, Haoyu; Wang, Zhe; Lu, Kangle; Wang, Ling; Zhang, Chunxiao; Li, Xueshan; Ma, Ruijuan; Song, Kai
After 56 days of culture experiment, spotted seabass (Lateolabrax japonicus) (10 fish per tank) fed diets with similar dietary iron levels (63, 188, and 554 mg/kg) were subjected to a 24-hour nitrite stress challenge at 27 °C and 33 °C, respectively. Nitrite stress significantly reduced the levels of hematocrit (HCT), red blood cell (RBC) and hemoglobin (Hb). The reduction in the 33 ℃ group was greater than that in the 27 ℃ group, and the decrease in the 63 mg/kg iron level group was the largest. The main impact analysis revealed that when the dietary iron level rose from 63 mg/kg to 554 mg/kg, the contents of red blood cell (RBC), white blood cell (WBC), and hemoglobin (Hb) in the blood and the activities of total antioxidant capacity (T-AOC) and catalase (CAT) in the serum significantly increased (P< 0.05). The group with an iron level of 188 mg/kg showed a considerably lower serum malondialdehyde (MDA) content than the group with an iron level of 63 mg/kg (P< 0.05). The 63 mg/kg iron level group observed substantial decreases (P< 0.05) in hemoglobin (Hb) and total antioxidant capacity (T-AOC) under 27 °C, but dietary 188 and 554 mg/kg iron mitigated this phenomenon. Iron deficiency and high temperatures facilitated the formation of methemoglobin (MetHb). This study demonstrated that spotted seabass is more susceptible to the physiological harm caused by nitrite at 33 ℃ than at 27 ℃. Iron deficiency exacerbated the risk of hemocytopenia, oxidative stress and hypoxia. Dietary iron fortification could enhance tolerance by enhancing antioxidant capacity and blood homeostasis.
Factors Influencing Consumer Willingness to Consume Giant River Prawn (Macrobrachium rosenbergii): An Empirical Analysis in Jiangsu Province, China
(2025) Lei, Jingjing; Li, Fei; Xu, Shiwei; Gao, Qiang; Jin, Hongtao; Deng, Zhikang
Against the backdrop of global food security strategies and the construction of a Diversified Food Supply System, giant river prawn (Macrobrachium rosenbergii), a key freshwater aquaculture species in China, plays a critical role in ensuring the supply of high-quality protein through industrial development. Using the Theory of Planned Behavior (TPB) as the theoretical framework, this study empirically examines the factors influencing consumers’ intention to consume Macrobrachium rosenbergii based on a questionnaire survey of 352 respondents in Jiangsu Province, China, employing a binary logistic regression model. The results reveal that product attributes (β = 0.846, OR = 2.331), health awareness (β = 0.797, OR = 2.218), behavioral attitude (β = 0.556, OR = 1.743), subjective norms (β = 0.476, OR = 1.610), and perceived behavioral control (β = 0.780, OR = 2.182) significantly and positively affect consumption intention, while households with elderly members or children (β = -1.674, OR = 0.187) and low-income families (β = -2.897, OR = 0.055) exhibit a significant negative impact. Based on these findings, the study proposes designing price-friendly and convenient consumption solutions for low-income groups, strengthening communication about product attributes and health benefits to enhance consumer recognition, and optimizing the industrial chain and distribution channel layout to improve market accessibility and purchasing convenience, aiming to establish a consumer-driven development model for the giant river prawn industry and promote its integration into the national diversified food supply system.
The long-term effect of non-invasive sampling on the genetic diversity and growth performance of cultured Gymnocypris chilianensis (Cyprinidae) population: an evaluation based on 70 days
(2025) Liu, Biyuan; Cheng, Qiqun; Lou, Zhongyu; Peng, Di; Qin, Yong; Song, Dan
Non-invasive and non-lethal sampling methods are widely employed in conservation biology, but their long-term effects on sampled organisms remain understudied. To evaluate these impacts on fish and establish a scientific framework for ethical sampling practices, we conducted a dual investigation using the plateau-endemic species Gymnocypris chilianensis (Cyprinidae). First, genetic diversity in a cultured population was analyzed using non-invasive mucus sampling. Sequencing of mitochondrial Cytb (1098 bp) and D-loop (696 bp) regions revealed low genetic diversity, with Cytb showing six polymorphic sites, seven haplotypes (haplotype diversity H = 0.354; nucleotide diversity π = 0.0004), and D-loop displaying 10 polymorphic sites, 9 haplotypes (H = 0.787; π = 0.00315). The D-loop region demonstrated higher variability, supporting its utility as a marker for population genetic studies. Second, 180 individuals were divided into three groups, i.e., groups N (macus), Q (fin), and C (control), and monitored for 70 days to assess growth parameters (standard length, body weight), condition factor (K), and mortality. Generalized additive models (GAMs) revealed no significant differences in morphological indicators (p > 0.05) or mortality rates among groups, with a nonlinear standard length–weight regression relationship identified. Notably, all groups exhibited K values exceeding 200%, indicating excellent body condition. Comparative analyses confirmed minimal sequence divergence between non-invasive and traditional sampling methods, validating methodological reliability. This study provides the first long-term evaluation of non-invasive and non-lethal sampling impacts on fish growth, the results showed that non-invasive sampling had no significant impact on the growth or survival of fish, which was very important for future research and protection, combined with genetic diversity insights, offering critical guidelines for conserving vulnerable species like G. chilianensis in the Qinghai-Tibet Plateau.
Comparison of the potential effects of three compound probiotics on growth, intestinal microbiota, intestinal morphology, and immunity of juvenile turbot (Scophthalmus maximus)
(2025) Xu, Hanzhi; Zhao, Xiaowei; Huang, Hua; Chen, Kun; Cao, Yanan; Wang, Han; Liu, Xiaodan; Wang, He; Li, Zhanjun
A 36-day feeding trial was conducted to compare the effects of the diet addition of three commercial compound probiotics (Ma, Mb, Mc) on growth performance, intestinal health, and immunity of juvenile turbot (30-day-old, 0.15 ± 0.07 g). The results showed that adding probiotics to the diet can improve growth performance and the Ma and Mb group had the most significant facilitation effect (P < 0.05). Based on 16S rRNA sequencing, the effects of different probiotic groups on the intestinal microbiota were explored. Ma group increased OTUs richness and diversity, the relative abundance of Firmicutes and Bacteroidetes at the phylum level, and Lactobacillus at the genus level, with the significant enrichment of potential probiotics. Notably, the Ma group also significantly increases metabolism-related functions. Both probiotic groups increased the fish intestinal villus length. Moreover, after 14 days of challenge test with Aeromonas salmonicida, the cumulative mortality rate was the lowest and the time of death was the latest in the Ma group. In general, compared with the Mb and Mc group, supplementation of Ma to juvenile turbot could significantly improve growth, intestinal morphology, and immune ability with the promotion of potential probiotics in the host intestine.
The Role of Aquaculture and Fisheries in EU27 Food Security: A Comparative Analysis of EU13 and EU14 States
(2025) Alsaleh, Mohd; Abdul-Rahim, A.S.
Ocean-sourced foods are essential for providing food security, putting an end to starvation, and building healthy, environmentally friendly, and resilient food systems. Still, it is important to keep these in mind while discussing food. More money was made for developing countries by exporting blue foods than by exporting any other agricultural products combined. The European Union Region (EU27) has countries with diverse economic structures, from highly developed industrial economies in older members to those in Central and Eastern Europe still catching up. Splitting them allows for in-depth study of economic structures, models, and growth mechanisms, and can inform targeted policy recommendations for growth and convergence. The core objective of this study is to analyse how aquaculture and fisheries production impact food security in the EU2 members based on their economic structure development; European Union Developing State (EU13) and European Union Developed State (EU14) countries from 1990 to 2023. To address potential endogeneity issues, robust least squares (RLS), two-stage least squares (2SLS), and ordinary least squares (OLS) estimators were employed, leading to significant findings. The findings confirm the existing knowledge and indicate that the role of aquaculture and fisheries production in ensuring food security is more pronounced in developing EU13 countries compared to wealthier EU14 countries. Aligning with existing knowledge, the analysis reveals that factors such as gross domestic product (GDP) and governance play a more crucial role in ensuring sustainable food security in developing EU13 countries relative to their wealthier EU14 counterparts. The reliance on fossil fuels has a more pronounced impact on food insecurity in developed EU14 nations compared to developing EU13 countries. This study suggests that policymakers in the EU14 developed countries provide policies targeted at promoting the growth of aquaculture production and fisheries production top priority based on the research conclusions. Additionally, this study suggests that policymakers in the industrialized EU14 countries improve governance, aquaculture economics, fisheries economics, and the efficiency of fossil fuel usage.
Development of a microparticulate feed for the nursery rearing of the giant mottled eel (Anguilla marmorata)
(2025) Huervana, Fredson H.; Nodque, Kelee Ira B.; Nantong, Cedric Jay A.; Guyapale, Rizza Mae T.; Traifalgar, Rex Ferdinand M.
This study was conducted to develop a microparticulate feed for the nursery culture of Anguilla marmorata. The developed crumble-type formulated feed was evaluated through two separate feeding trials. In the first trial, the growth performance of A. marmorata elvers fed with dry crumble-type feed was compared to those fed the conventional moist dough-type nursery diet over a 16-day period. The results revealed that elvers fed with the crumble-type feed exhibited significant growth performance, with a specific growth rate (SGR) of 1.57 and percent weight gain (%WG) of 24.62%, (p<0.05). No significant difference in survival was observed, as both dietary treatments achieved a 100% survival rate. Additionally, better water quality was observed in tanks fed with the crumble-type feed as compared to the dough-type. In the second trial, the applicability of the crumble-type feed to support elvers growth was tested on a larger scale rearing system for 120 days. Results demonstrated excellent growth and survival, with elvers exhibited an SGR of 1.52, %WG of 290.47%, and survival of 86.50%. Findings from this study underscore the successful development of a microparticulate feed that supports optimum growth and survival of A. marmorata elvers, while offering practical advantages in terms of ease of management and feed preparation.
Positive relationship between Clostridium abundance and bacterial community diversity in the aquaculture water of hybrid grouper (*Epinephelus fuscoguttatus*♀ × *E. lanceolatus*♂) in coastal areas of Hainan Island
(2025) Zheng, Minjing; Li, Zhen; Chen, Yonggan
The hybrid grouper, characterized by its accelerated growth rate, robust disease resistance, and superior nutritional-economic value, has emerged as a pivotal mariculture species in China and Southeast Asia. This study systematically revealed the microbial communities within aquaculture waters across five coastal regions of Wenchang, Wanning, Lingshui, Ledong, and Dongfang in Hainan Island, and elucidated potential interspecific interactions in microbiota. The α-diversity indices, principal coordinate analysis and β-diversity metrics demonstrated substantial variations in bacterial abundance, species diversity, and community structure. Conversely, fungal assemblages showed no statistically significant spatial differentiation. Taxonomic profiling identified Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, and Cyanobacteria as dominant bacterial phyla. Fungal communities were predominantly composed of Ascomycota and Basidiomycota. Notably, Clostridium abundance in the outdoor mariculture ponds exhibited positive correlations with key bacterial parameters, suggesting its potential as a bioindicator for microbial community dynamics. These findings advocate for precision management strategies when utilizing Clostridium-based probiotics in outdoor mariculture systems. We propose implementing a dose-response protocol to optimize probiotic supplementation thresholds and prevent dysbiosis-induced fluctuations in aquatic microbial diversity. This approach provides critical insights for microbiome engineering in industrialized grouper aquaculture systems.
Molecular characterization and gene expression profile of Foxl2 in the Cherax quadricarinatus
(2025) Zhang, Yucheng; Zheng, Jianbo; Zhou, Yangda; Li, Fei; Liu, Shili; Jiang, Wenping; Chi, Meili; Cheng, Shun; Zhang, Haiqi
Foxl2 (Forkhead box protein L2), a forkhead transcription factor, plays a pivotal role in the ovarian development of aquatic species. However, the biological function of Foxl2 in Cherax quadricarinatus remains underexplored. In this study, the full-length cDNA of Cq-Foxl2, comprising 2,325 bp, was characterized, with a 1,695 bp open reading frame (ORF) encoding a protein of 564 amino acids. Bioinformatics analysis revealed that the predicted amino acid sequence of Cq-Foxl2 contained a conserved forkhead box domain, exhibiting high homology with other crustaceans, particularly Procambarus clarkii. qRT-PCR analysis indicated that Cq-Foxl2 was predominantly expressed in the ovary, with minimal detection in other tissues. Expression levels of Cq-Foxl2 were abundant throughout different stages of ovarian development. Moreover, RNAi-mediated silencing of Cq-Foxl2 led to a significant reduction in the transcription of Cq-Dsx and a decrease in the gonadosomatic index of female C. quadricarinatus. These findings offer essential insights for further exploration of the role of Cq-Foxl2 in ovarian development.
Effects of densities and alkalinities on the rearing performance of all-male giant freshwater prawns (Macrobrachium rosenbergii De Man, 1879) postlarvae
(2025) Huong, Huynh K.; Vu, Le H.; Nhi, Nguyen T.H.; Diep, Doan X.
Farming all-male giant freshwater prawns (Macrobrachium rosenbergii) is becoming increasingly common in aquaculture due to the faster growth and larger maximum weights in males than females. However, the key factors influencing the development of all-male giant freshwater prawns (post-larval AMGFPs) under captive conditions remain insufficiently explored. This study evaluated the growth and survival of post-larval AMGFPs under varying stocking densities (200–600 ind m^−3^) and alkalinities (80--160 mg CaCO~3~ L^−1^) across two completely randomized experiments in three replicates in 1 m^3^ tank systems with a biofloc environment, where a carbon source (rice flour with a C:N ratio of 15:1) was added for biofloc formation. Post-larval AMGFPs (initial mean weight and length of 0.012-0.016 g and 1.13-1.53 cm, respectively) were maintained at a salinity 5‰ and fed commercial feed (40% crude protein) for 60 days. The stocking density in Experiment 2 was 500 m^−3^ (the best stocking density obtained in Experiment 1). The results showed that after 45 rearing days under the experimental conditions, all growth parameters, such as mean weight (MW), daily weight gain (DWG), specific growth rate in weight (SGRw), mean length (ML), daily length gain (DLG), and specific growth rate in length (SGRL), increased as stocking density rose within 200-500 ind m^−3^ but declined at 600 ind m^−3^. The highest survival rate (SR) was observed at a density of 500 ind m^−3^, which was significantly higher than at other densities. Additionally, the highest final biomass (FB) was in 500 and 600 ind m^−3^ and differed from other densities. Besides, from rearing day 30 onward, within the alkalinity range of 80–160 mg CaCO~3~ L^−1^, all growth parameters (weight and length), SR, and FB increased in proportion to both alkalinity level and rearing time, with the highest values at 160 mg CaCO~3~ L^−1^ and significantly differing from those at lower levels at the experimental end. A stocking density of 500 ind m^−3^ and an alkalinity of 160 mg CaCO~3~ L^−1^ were recommended as optimal for rearing post-larval AMGFPs under the tested conditions.
Effects of Aquaculture Substrate on Key Aquaculture Environmental Parameters, Growth Performance, and Physiological Responses in Babylonia areolata
(2025) Zhou, Cheng; Zhao, Wang; Yang, Rui; Huang, Junhua; Qin, Haipeng; Ma, Zhenhua; Wang, Xiaoyu
To enhance the aquaculture environment of Babylonia areolata, this study evaluated five substrates—small ceramsite, medium ceramsite, large ceramsite, quartz sand, and river sand (control)—on water quality, growth, survival, digestive enzyme activity, and antioxidant capacity in juvenile B. areolata. Results demonstrated that small ceramsite achieved optimal performance in water quality maintenance, survival rate (92.4 ± 3.1%), and growth rate (specific growth rate: 1.85 ± 0.12%/day). Small ceramsite significantly enhanced lipase (18.7 ± 1.2 U/mg protein) and trypsin (23.4 ± 1.5 U/mg protein) activities in hepatopancreas compared to control (p < 0.05). In contrast, large ceramsite exhibited lower digestive enzyme activity than control (p < 0.05). Glutathione peroxidase (GSH-PX) activity in large ceramsite groups declined significantly (p < 0.05), while lysozyme activity in hepatopancreas surpassed that in foot and mantle across all substrates. Conversely, acidic phosphatase (ACP) activity in mantle and hepatopancreas under large ceramsite was significantly lower than in foot tissues (p < 0.05). Substrate type critically influences growth, immune function, and antioxidant capacity in B. areolata. Small ceramsite outperformed others by improving water quality, enhancing survival, and accelerating growth, while simultaneously boosting digestive and antioxidant enzyme activities. These findings suggest small ceramsite as the optimal substrate for B. areolata aquaculture, offering a strategy to optimize rearing conditions, improve economic efficiency, and promote mollusk health.
Assessment of Indigenous Feed Ingredients on Growth and Feed Utilization Efficiency in Juvenile Milkfish (Chanos chanos Forsskål, 1775)
(2025) Macusi, Edison D.; Sales, Anthony C.; Jimenez, John Edward M.; Vender, Ethel Kate E.; Bongas, Henzel P.; Macusi, Erna S.; Andam, Michael B.
This study aimed to assess the feasibility of utilizing indigenous feed ingredients for milkfish (Chanos chanos Forsskål 1775) aquaculture. Nine different feed treatments were tested: taro, golden apple snail, azolla plant, blood meal, cacao pod, banana stem, copra meal, soybean meal, and a control. We used 15 juvenile milkfish (initial weights=6.4-7.4 g; initial lengths=5.3-5.7 cm) cultured in 80 L tanks and replicated three times to assess their growth and feed utilization. After 60 days of culture, copra meal and Azolla attained 23.0 g and 21.8 g, which were higher compared to the other treatments. This was followed by taro (18.9 g), bloodmeal (18.4 g), golden apple snail (18.2 g), control (18.2 g), cacao pod (18.2 g), soybean treatments (17.5 g), and lastly banana stem (16.1 g). Comparison of the initial length and weight of the treatments versus their final values during the 60 days of culture showed differences (length: t=20.0, p=0.0001, df=16; weight: t=16.5, p=0.0001, df=16) but none between the nine treatments (p\>0.05). In terms of average daily weight gain, copra meal gained faster (0.34 g), followed by Azolla (0.24 g), and then the control (0.22 g) and bloodmeal (0.19 g) with banana stem the last (0.15 g). While in terms of feed conversion ratio (FCR), copra meal (1.1), azolla (1.3), taro (1.5) and the control (1.5) had the lowest FCR values; banana stem (1.9) and soybean meal treatments (2.0) had the highest. Fulton's condition factor showed that these four treatments (taro=1; Azolla=1.2; copra meal=1.2; control=1.2) were closest to 1, signifying a healthy condition. The gonadosomatic index (GSI=0.005) and hepatosomatic index (HSI=0.03) values were consistent in the various treatments, showing no adverse effects suffered during the period of culture. The alternative feeds can be used in milkfish aquaculture to reduce the cost of operation.
Strengthening the Resilience of Aquaculture Competitiveness in EU Economies: An empirical analysis
(2025) Alsaleh, Mohd; Abdul-Rahim, A.S.
The European Union (EU27) faces significant challenges in enhancing the global competitiveness of its aquaculture market, such as stagnant production, high production costs, competition from cheaper imports, focus on sustainability, and consumer preferences. Thus, the main goal of this research is to explore how factors related to global competitiveness influenced the growth of the aquaculture sector in the EU27 from 1990 to 2023. The methods of ordinary least squares (OLS), two-stage least squares (2SLS), and robust least squares (RLS) estimations were used to address the endogeneity problem to arrive at the most important results. The effect sizes indicate that the system of innovation and human capital have a greater influence on the development of the aquaculture sector in the highly developed EU14 countries compared to the developing EU13 nations. The exact outcomes of the applied methods, however, demonstrate that the aquaculture market's expansion in EU13 developing nations is more influenced by market size, institutional quality, and economic growth compared to EU14 members. This study recommends that the EU27 region emphasize policy adjustments to balance sustainability with competitiveness, which includes streamlining regulations, improving access to financing, and fostering innovation in aquaculture technologies.
Development and Utilization of Transcriptome SSR Markers in Procambarus clarkii
(2025) Zeng, Dan; Zhang, Yunsheng; Du, Min; Shao, Liye; Xia, Hu; Han, Qing
To accelerate the research process of molecular marker-assisted breeding in Procambarus clarkii, this study conducted transcriptome sequencing of P. clarkii muscle tissue using the Illumina NovaSeq platform. Microsatellites (SSRs) were analyzed for distribution and sequence characteristics using MIcroSAtellite (MISA), and genetic diversity was studied in seven cultured populations. The results showed that the sequencing yielded between 9.9 to 12.7 Gb of clean data, and after assembly, produced between 52,244 to 83,367 contigs, identifying a total of 11,304 SSR loci. The SSRs were predominantly dinucleotide and trinucleotide repeats, accounting for 33% and 27% of the total loci, respectively. The average observed heterozygosity (Ho) across the seven different regional aquaculture populations ranged from 0.374 to 0.502, while the average expected heterozygosity (He) ranged from 0.502 to 0.604. The average polymorphic information content (PIC) for the populations was greater than 0.450, indicating substantial genetic variability. According to Nei's genetic distance, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster tree divided the seven different regional aquaculture populations into two major clades: one comprising Yunnan (YN) and Qianjiang (QJ) and the other comprising the remaining five populations. The study found that 93.52% of the variation originated within populations, with only 6.48% of genetic variation attributable to differences between populations. These results lay a foundation for further assessment of the genetic diversity of P. clarkii, innovative utilization of germplasm resources, and in-depth studies into its molecular genetics and evolutionary mechanisms.
Research on ecological-economic value equilibrium path of aquatic products from trust perspective
(2025) Dai, Yunyun; Wang, Zhixiao; Liu, Qiyao; Pan, Yuting; Yang, Hong
Eco-aquatic products have two important attributes: food safety and eco-friendliness. However, in China, the market price of most eco-aquatic products is not significantly different from that of ordinary aquatic products, which fails to fully realize their ecological value and high quality. This paper analyzed the path of realizing the ecological value and economic value of aquatic products from the perspective of trust. Through the analysis of three cases of Suzhou Haimei Family Farm, Suzhou Pinwei Aquatic Products Company, and Jiangsu Yunian Agriculture and Forestry Ecological Development Company, this paper showed how to improve consumer trust through community participation, social network and government endorsement, aimed at maximizing the value of ecological aquatic products. The case analysis demonstrates that adhering to the concept of ecological priority and sustainable development, building regional or product brand, maintaining brand image, and innovating diversified business strategies can effectively promote the development of aquaculture industry to a healthier and more environmentally friendly direction, and achieve a win-win situation of economic and ecological benefits.
Research on the resilience level of fishery economy in coastal areas of China
(2025) Chen, Yingjie; Ma, Teng; Xu, Shiwei; Zhao, Yu; Deng, Zhikang
Multiple risks such as environmental pollution, market risk, and extreme weather pose severe challenges to fishery production, fishermen 's lives and property, and the sustainable development of fishery. Improving the resilience of fishery economy is an inevitable requirement for coping with fishery production risks and achieving high-quality development of fishery economy. Therefore, based on the three dimensions of resistance and recovery ability, adaptation and adjustment ability, innovation and transformation ability, this study constructs a comprehensive index evaluation system of fishery economic resilience, and uses entropy method and Theil index method to measure the level of fishery economic resilience and regional differences in coastal areas of China from 2016 to 2023. The conclusions are as follows: (1) From 2016 to 2023, the level of fishery economic resilience in coastal areas of China showed a fluctuating growth trend of 'first decline and then rise', with an average value of 0.2566. (2) There are significant differences in the level of fishery resilience in different coastal areas. From 2016 to 2023, Shandong Province had the highest level of fishery economic resilience (0.5226), and Hainan Province had the lowest level of fishery economic resilience (0.0751). The eastern marine economic circle has the highest fishery economic resilience (0.2713), followed by the southern marine economic circle (0.2650), and the northern marine economic circle has the lowest fishery economic resilience (0.2371). (4) The regional differences in the level of fishery economic resilience in coastal areas of China mainly come from inter-group differences, with a contribution rate of 68.36 %. The difference in the level of fishery economic resilience between the three major marine economic circles has gradually become the most important factor affecting the difference in the level of fishery economic resilience in China.
Identification and characterization of pathogenic Aeromonas hydrophila isolated from clinically diseased Chinese rice-field eel, Monopterus albus
(2025) Jiang, Jingwen; Xue, Mingyang; Liu, Wenzhi; Jiang, Nan; Zhou, Yong; Liu, Yisha; Li, Mengmeng; Zhong, Qiwang; Fan, Yuding
Aeromonas hydrophila is recognized as a prevalent pathogenic bacterium in aquaculture. An outbreak of a disease characterized by a high mortality rate has been reported at a Chinese rice-field fish farm (Monopterus albus) located in Hubei Province. This study isolated a causative A. hydrophila strain (YFI-C1) in diseased Monopterus albus through bacterial isolation and subsequent morphological, physiological, biochemical, evolutionary and molecular biology analysis. Ten virulence genes, including those that encode cytotoxic enterotoxins (act and alt), a quorum sensing-controlled virulence factor (LuxS), a temperature-sensitive protease (eprCAI), serine proteinase (ser), haemolysin-aerolysin (aeraA), nuclease (nuc), hemolysin (hlyA), lipase (lip), flagellin (fla) have been shown to be responsible for YFI-C1's pathogenicity. An artificial infection was utilized to evaluate the pathogenicity of YFI-C1, with a median lethal dose (LD50) of 1 × 106 colony forming units (CFU)/mL. The Kirby-Bauer test was used to measure YFI-C1's sensitivity to ten antibiotics. According to the findings, YFI-C1 was resistant to Cefothiophene but extremely susceptible to six antibiotics: Ciprofloxacin, Trimethoprim-sulfamethoxazole, Doxycycline, Enrofloxacin, Compound sulfamethoxazoles, and Flufenicol. The results of this study offer valuable insights for future research on bacterial disease prevention and treatment in Monopterus albus.
Effects of dietary marine red yeast on growth performance, digestive enzyme activities, antioxidant capacity, and immunity response of white-spotted conger (Conger myriaster)
(2025) Wang, Yuyu; An, Meiling; Shen, Hengxia; Chen, Tong; Li, Yuanyuan; Guo, Jianjun; Dong, Yanjun; Teng, Fuhai; Wang, Junxia; Hu, Ming
This study aimed to investigate the effects of marine red yeast (MRY) on growth performance, digestive enzyme activities, serum biochemistry, antioxidant capacity, immunological indices, and expression of immune-related genes in white-spotted conger (Conger myriaster). Four diets were supplemented with varying levels of MRY (0%, 0.5%, 1.0%, and 1.5%, designated as MRY0, MRY0.5, MRY1, and MRY1.5, respectively). Fish (average weight, 46.27 g) were divided into 12 net cages, with 3 cages per diet. The fish were fed diets twice daily for 56 days. Results indicated that dietary supplementation of MRY had no significant effects on growth and digestive enzyme activities (P\>0.05). Fish fed diets MRY1 and MRY1.5 had lower serum alanine aminotransferase (ALT) content than the MRY0 and MRY0.5 groups (P<0.05). Serum glucose (GLU) content showed an increasing trend with increasing MRY levels, and the MRY1.5 group had the highest value (P<0.05), while cholesterol (TC) and triglyceride (TG) levels exhibited an opposite trend, and fish-fed diet MRY1 had lowest values (P<0.05). Fish fed diet MRY0.5 had significantly higher hepatic glutathione peroxidase (GSH-Px) activity than those fed diets MRY0 and MRY1.5 (P<0.05). Hepatic total superoxide dismutase (T-SOD) and serum alkaline phosphatase (ALP) activities increased with the increase of dietary MRY level (P<0.05). Fish fed diet MRY1.5 exhibited significantly lower relative expression of heat shock protein 70 (HSP70) in the liver than fish fed diets MRY0 and MRY0.5 (P<0.05), and no significant difference was found in the relative expression of HSP90 in the liver among all groups (P\>0.05). In conclusion, 1%--1.5% MRY could be applied as a feed additive to effectively enhance the immune response of white-spotted conger.
Cyclophilin A in Megalobrama amblycephala: Molecular characterization and expression analysis in response to Aeromonas hydrophila challenge
(2025) Lu, Juanjuan; Xia, Hu; Gong, Yanan; Chen, Fuyan; Bu, Jianchao; Yang, Pinhong
Cyclophilin A (CypA) is a cell’s primary receptor of Cyclosporin A (CsA). It is essential for biodiversity and plays an important role in the immune system. In this study, the full-length cDNA sequence (GenBank ID: PV256473) of Megalobrama amblycephala CypA was acquired by PCR and RACE technology. The complete cDNA sequence of M. amblycephala CypA is 901 bp. The lengths of the 5′ untranslated region (UTR), the 3′ untranslated region, and the open reading frame (ORF) are 77 bp, 329 bp, and 495 bp, respectively. It encodes a total of 164 amino acids. The theoretical isoelectric point of M. amblycephala CypA is 8.6, and the relative molecular weight is 17.43 KDa. According to homologous sequence retrieval in GenBank, the similarity between CypA amino acid sequence of M. amblycephala and other fish was Cyprinus carpio (94.51%), Ctenopharynodon idellus (92.07%), Carassius gibelio (92.07%), Labeo rohita (92.07%), Danio rerio (91.46%), and Onychostoma macrolepis (91.46%). ESyPred3D predicted that the CypA of M. amblycephala contained 8 β-pleated sheets and double α-helixes, which might form the active central region of CypA. MEGA 5.1 software was used for multiple sequence comparison analysis, and the adjacency method constructed the genetic phylogenetic tree. It was found that the CypA gene of M. amblycephala was clustered into one branch of Cyprinidae fish CypA, and CypA of M. amblycephala was closely related to CypA of Cyprinus carpio. Expressions of CypA in different tissues of M. amblycephala were detected through qPCR; it was found that the expression level of the CypA gene was highest in the head kidney, followed by liver, kidney, and spleen. The M. amblycephala was immersed in Aeromonas hydrophila for infection; it was found that expression levels of CypA in head kidney, liver, gills, and intestines first increased, then decreased in the first 72 h of infection. Specifically, the expression levels reached a peak at 6h in the head kidney, at 12 h in the liver and gills, and at 24h in the intestines. It indicated that the expression of M. amblycephala CypA gene could be significantly induced after A. hydrophila infection—expression levels of CypA in the head kidney peak earlier than in the liver and intestines. Moreover, the relative expression level of CypA in the head kidney at peak (15.02-fold) is significantly higher than that in the liver (6.89-fold), intestines (9.92-fold), and gills (7.42-fold). It is suggested that CypA might play an essential role in the head kidney of M. amblycephala in defense against bacterial infection.

Browse

Recent Submissions