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Title: Effects of molting on the growth of primary cultured muscle cells from and Short Communication 
Author: Wang, Wei-Na; Wang, An-Li; Nu, Dong-Hong; Liu, Yi-Chen; Sun, Ru-Yong
Date: 2005
Publisher: Israeli Journal of Aquaculture - BAMIGDEH
Citation: Wang, W., Wang, A., Nu, D., Liu, Y., & Sun, R. (2005). Effects of molting on the growth of primary cultured muscle cells from and Short Communication. The Israeli Journal of Aquaculture - Bamidgeh, 57(4), 264-270.
Abstract: This study examined the effects of molting stage on the growth of primary-cultured cells from the shrimps Macrobrachium nipponense and Penaeus vannamei. Muscle cells in different molting stages were cultured in L-15 medium, supplemented with 1g/l glucose,1g/l MgCl2,1 g/l CaCl2,100 IU/ml penicillin, and 100 μg/ml streptomycin. Results showed that cells cultured in the early postmolt stage grew best. They had an unfolding cell shape, a compact monolayer, a high rate of coverage, and the highest RNA:DNA ratio. Differences among early postmolt, premolt, and intermolt cells were significant (p<0.05).
Series/Report No.: The Israeli Journal of Aquaculture - Bamidgeh
Pages/Duration: 7 pages
ISSN: 0792-156X
Keywords: cell culture, Macrobrachium nipponense, molt, Penaeus vannamei, RNA:DNA ratio
LC Subject Headings: Fish culture--Israel--Periodicals.
Fish culture--Periodicals.

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