Antibacterial characterization of Bacillus velezensis LG37 and mining of genes related to biosynthesis of antibacterial substances

dc.contributor.author Liu, Guangxin
dc.contributor.author Deng, Yiqin
dc.contributor.author cheng, Changhong
dc.contributor.author Ma, Hongling
dc.contributor.author Jiang, Jianjun
dc.contributor.author Feng, Juan
dc.contributor.author Guo, Zhixun
dc.date.accessioned 2022-12-07T19:29:52Z
dc.date.available 2022-12-07T19:29:52Z
dc.date.issued 2022
dc.description.abstract Bacillus velezensis LG37 secretes various antibacterial substances and inhibits the growth of other bacteria. Here, we analyzed the antibacterial characteristics and the screening and verification of genes related to the synthesis of the antibacterial substance of LG37 by antibacterial activities experiment, Local BLAST+, and RT-PCR. LG37 was isolated from aquaculture water and preserved in our laboratory. The phylogenetic tree was used to analyze the genetic relationship between LG37 and the bacteriostatic test indicator strain. LG37 had a more substantial inhibitory effect on closely related strains, while the inhibitory effect on the more distantly related strains was weak. Combined with the results of genome sequencing, the ribosomal peptide (RP) bacteriocin gene and non-ribosomal peptide synthetase (NRPSs) related gene clusters were screened and analyzed. A total of six gene-coding RP bacteriocins and two genes coding surfactins and fengycin A NRPSs gene cluster were screened. Local BLAST+ analysis revealed a total of 11 NRPSs gene clusters. The active expression of the NRPSs and RP encoding genes was further validated by RT-PCR. The findings revealed various genes and gene clusters encoding RP bacteriocins and NRPSs in B. velezensis LG37. The bacterium is potentially valuable in diverse applications in aquaculture.
dc.format.extent 12 pages
dc.identifier.doi https://doi.org/10.46989/001c.57533
dc.identifier.issn 0792-156X
dc.identifier.uri https://hdl.handle.net/10524/68319
dc.relation.ispartof The Israeli Journal of Aquaculture - Bamidgeh
dc.subject Bacillus velezensis
dc.subject bacteriocin
dc.subject gene cluster
dc.subject non-ribosomal peptide
dc.subject ribosomal peptide
dc.title Antibacterial characterization of Bacillus velezensis LG37 and mining of genes related to biosynthesis of antibacterial substances
dc.type Article
dc.type.dcmi Text
prism.volume 74
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